Background: Oral cancer is often associated with poor prognosis and it is found that conventional treatment options cause severe side effects, adjacent tissue disfigurement, and loss of function. Recently, silver nanoparticles (AgNPs) paved their path for cancer treatment. Aim: This study aimed to investigate cytotoxic effects of fungal procured AgNPs on oral squamous cell carcinoma (SCC-9) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methodology: The silver nanoparticles were biosynthesized using the fungi Fusarium semitectum. Cell lines were cultured in a 1:1 ratio of Dulbecco's Modified Eagle's medium and Ham's F12 medium and subcultured in a T-75 cm2 flask. Cell count was adjusted to 1 × 105 cells/ml; 50,000 cells/well were seeded into a 96 well plate and incubated at 37°C, for 24 h in 5% CO2 humidified conditions. AgNPs (1.75–50 μl/ml) were added to the plates and further incubated at 37°C for another 24 h. Medium containing cells without AgNPs were used as a control group. Later, 20 μl of MTT was added to each well and incubated for 6 h at above-mentioned conditions. About 0.1 mL of Dulbecco's Modified Eagle's Medium solution was added to each well to solubilize formazan. The absorbance was measured using a Tecan reader at 540 nm. The experiment was repeated thrice independently. The percentage (%) inhibition of growth and the AgNP's concentration that prevents the cell growth by 50% (IC50) were determined. Results: Significant dose-dependent inhibition of the growth of SCC-9 cell lines was seen and IC50 was found at 12 μl/ml concentration of AgNPs. Conclusion: Biosynthesized AgNPs of fungal origin exhibit effective anticancer properties against the SCC-9 cell line.

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Aim: Using N acetyl cysteine (NAC) and Chlorhexidine as final irrigants, the study aims to examine the push out bond strength of distinct sealers. Materials and Methods: Access and biomechanical preparation were done on decoronated 80 single-rooted teeth. Canals were alternatively irrigated using 5ml of 5.25% NaOCl and 5ml of 17% EDTA solution during instrumentation. On the basis of final irrigation procedure, the samples are divided into two groups: Group 1 – NAC and Group 2 – 2% Chlorhexidine. Using paper points, canals were dried and subdivided depending on the sealers used, Group 1A and 2A- AH plus and 1B and 2B- BioRoot RCS. Teeth were obturated and middle root sections were tested for push-out bond strength using universal testing equipment. Statistical analysis was done. Results: NAC with AH-plus sealer had the maximum push-out strength, whereas Chlorhexidine with BioRoot RCS sealer had the lowest. Conclusion: NAC increases the bond strength of the obturating materials as the final irrigant. NAC can be considered as a final irrigant for endodontic therapy.

Objective A retreatment is a conservative approach than periapical surgery in case of failed root canal treatment. Retreatment techniques that are efficient and faster should be the main concern to treat endodontic failure. This study aimed to compare the time required by various hand and rotary retreatment systems for the retrieval of gutta-percha (GP) from root canals. Materials and Methods A total of 120 extracted single-rooted premolar teeth with straight canals were chosen. Biomechanical preparation was completed using the Step-Back approach keeping the master apical file size 35. Then, teeth were down-packed and back-filled with System B device, along with AH Plus sealer. The teeth were categorized into six groups (n = 20) at random as per techniques applied for retrieval of GP: group I hand instrumentation (Hedstrom files and Gates-Glidden drills); group II ProTaper Universal Retreatment (PTUR) system; group III Mtwo Retreatment (Mtwo R) system; group IV D-RaCe; group V R-Endo; and group VI Mani Gutta Percha Remover (NRT-GPR). A chronometer was used to measure the time required for retrieval of GP. Data were examined by comparative evaluation of one-way analysis of variance and chi-square test. Results Among all the retreatment techniques used, D-RaCe was faster in GP retrieval than other groups. NRT-GPR system also took less time than other groups except for the D-RaCe system. The Mtwo R system was the slowest among all groups. Conclusion D-RaCe retreatment instruments required extensively less time for retrieval of GP than other groups (p < 0.001).

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