Background and objectives: Moderator band is a specialized bridge present between the base of the anterior papillary muscle and interventricular septum. It carries the right branch of the bundle of HIS with it. The band is known to prevent the over distension of the right ventricle during the diastolic phase. There is a need of lot of research and studies on the septomarginal trabecula as it proves to be important clinically. Here we measured the length, breadth, height, angle with the interventricular septum, and the superficial marking of Moderator band on the sternocostal surface of the right ventricle is done. This paper describes the morphological variations found in its origin and insertion.Materials and Methods: 15 formalin-soaked cadaveric hearts were taken from the Department of Anatomy of KVG Medical College, Sullia. The foetal hearts, the hearts with gross pathology, if any are excluded from the study. Result:The morphometric parameters are tabulated and the morphological variations are noted. The results are compared with the results of the previous studies on the moderator band. Conclusion:It is confirmed that the thickness of moderator band varies at its origin and insertion. The surface marking of moderator band on sternocostal surface of the heart will help in locating the moderator band through the echocardiography and during cardiac surgeries on right ventricle. The possibility of second moderator band, multiple origin or insertion is more than 20%.