Cadmium (Cd) is considered as a very toxic heavy metal which transfers to the body through many routes as food, water and air. Zinc (Zn) and selenium (Se) have a great role in alleviation of Cd toxicity in kidneys. This study aimed to look into the interaction between Cd, Zn, Se and their effects on gene expression of the antioxidant enzymes in the kidneys of rats. Fifty male adult albino rats were divided into five groups (n=10) that received orally the following doses daily for one month: Group I: control group received normal saline, Group II: received 2mg/kg BW CdCl 2 , Group ΠI: was administered 2mg/kg CdCl 2 and 2mg/kg ZnCl 2 , Group IV: received 2 mg/kg CdCl 2 and 0.23 mg/kg Na 2 Seo 3 , and Group V: received 2 mg/kg CdCl 2 , 2 mg/kg ZnCl 2 and 0.23 mg/kg Na 2 Seo 3 ). The gene expression levels of SOD1, CAT, GPx, HSP70 and MT1 were assessed in the kidneys; treatment with Cd lowered the expression of antioxidant enzymes (SOD1, CAT, and GPx) and increased the expression level of HSP70 and MT1. Only partial ameliorative effects on the oxidative stress caused by Cd in the kidney have been observed with Se or Zn supplementation during exposure to Cd, while the co-treated with Se and Zn revealed better protection against the observed oxidative stress in kidney.