Mitochondria-mediated apoptosis is a major mode of cell death and is inextricably linked to various pathological processes such as tumorigenesis. However, there is still a paucity of non-toxic tools that...

Abstract.Expounding the connotation of the core competence of construction consultant enterprises and establishing the evaluation index system about core competence of construction consultant enterprises from five aspects which are the management,technology,economic benefit,human resources and market development.Using entropy weight method to determine the evaluation indexes weight, then applying grey correlation method to evaluate and compare the core competence by four enterprises.

Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma cell. To study its effect on the proliferation and apoptosis of different tumor cells and verify its anti-tumor non-specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT assay, cell growth curve, TUNEL assay，flow cytometry，transmission electron microscopy(TEM), the biological activity of 19peptide was studied. Experiments in vitro identified that obtained 19peptide could inhibit proliferation of hela cell and hepatocarcinoma cell. It also could promote two tumor cells apoptosis. Its anti-tumor effect will lay foundation on its mechanism of action research and clinically tumor therapy in future.

7 peptide of Tumstatin(T-7 peptide) is composed of 185-191 amino acids. To study T-7 peptide antitumor activity to human non-small-cell carcinoma(A549), T-7 peptide was designed and synthesized by amino acid synthesizer. Its purity ran up to 98.45% by HPLC and MS. The effect of T-7 peptide on A549 cell growth was observed by MTT assay, growth curve and transmission electron microscopy(TEM). T-7 peptide had the effects of suppressing A549 cell growth and promoting its apoptosis, showing dose- and time-dependent. Its IC50 was 92.84 μg/ml. TEM also revealed that A549 cell treated with T-7 peptide appeared apoptotic morphology,such as cell pyknosis and mitochondrial vacuoles formed. While T-7 peptide had little effect on human umbilical vein endothelial cells(ECV304). These researches were significant to treat human non-small-cell carcinoma in the future.

To study the effect of biological activity of Tumstatin 7 peptide on the cell proliferation and apoptosis of B16 melanoma. Tumstatin 7 peptide was synthesized and its purity is 98.4532% by high-performance liquid chromatography. The effect of 7 peptide on B16 melanoma was observed by MTT, cell growth curve, the observation of the transmission electron microscope (TEM). 7 peptide can significantly inhibit B16 melanoma cell proliferation, showing dose- and time-dependent .Its IC50 was 72.53 μg/ml.The morphology of B16 melanoma cell was obviously changed by TEM, such as karyopyknosis and apoptotic bodies. So7 peptides can inhibit the proliferation of melanoma cells and promote melanoma cells apoptosis. It will be of great potential value to melanoma treatment.