A significant fraction of the nuclear DNA of all eukaryotes is comprised of simple sequence repeats (SSRs). Although these sequences are widely used for studying genetic variation, linkage mapping and evolution, little attention had been paid to the chromosomal distribution and cytogenetic diversity of these sequences. In this paper, we report the distribution characterization of mono-, di-, and tri-nucleotide SSRs in Brassica rapa ssp. chinensis. Fluorescence in situ hybridization was used to characterize the cytogenetic diversity of SSRs among morphotypes of B. rapa ssp. chinensis. The proportion of different SSR motifs varied among morphotypes of B. rapa ssp. chinensis, with tri-nucleotide SSRs being more prevalent in the genome of B. rapa ssp. chinensis. We determined the chromosomal locations of mono-, di-, and tri-nucleotide repeat loci. The results showed that the chromosomal distribution of SSRs in the different morphotypes is non-random and motif-dependent, and allowed us to characterize the relative variability in terms of SSR numbers and similar chromosomal distributions in centromeric/peri-centromeric heterochromatin. The differences between SSR repeats with respect to abundance and distribution indicate that SSRs are a driving force in the genomic evolution of B. rapa species. Our results provide a comprehensive view of the SSR sequence distribution and evolution for comparison among morphotypes B. rapa ssp. chinensis.
Due to that the Doppler parameters vary according to slant and the resolution is lower using imaging algorithm of traditional pulse compression in processing raw echo data of the missile-borne synthetic aperture radar (SAR). Moreover, an algorithm is proposed to solve these problems, which is based on the fractional Fourier transform (FrFT) for missile-borne SAR imaging. Firstly, an echo signal model is built for the terminal guidance stage of the missile-borne SAR. Secondly, measure the chirp rate of the echo signal through the local optimum processing and get the optimum angles for the FrFT, and then the entire SAR image can be obtained by using FrFT with the optimum angles of the azimuth and range. Finally, the performances of the algorithms are assessed using simulated and real Radarsat-1 data sets. The results confirm that the FrFT-based missile-borne SAR processing methods provide enhanced resolution yielding both lower side lobes effects and improved target detection. The method introduced in this paper has important theoretical significance in detection and recognition for military targets and precision guidance.
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