Background:Delivery room resuscitation assists preterm infants, especially extremely preterm infants (EPI) and extremely low birth weight infants (ELBWI), in breathing support, while it potentially exerts a negative impact on the lungs and outcomes of preterm infants. This study aimed to assess delivery room resuscitation and discharge outcomes of EPI and ELBWI in China.Methods:The clinical data of EPI (gestational age [GA] <28 weeks) and ELBWI (birth weight [BW] <1000 g), admitted within 72 h of birth in 33 neonatal intensive care units from five provinces and cities in North China between 2017 and 2018, were analyzed. The primary outcomes were delivery room resuscitation and risk factors for delivery room intubation (DRI). The secondary outcomes were survival rates, incidence of bronchopulmonary dysplasia (BPD), and risk factors for BPD.Results:A cohort of 952 preterm infants were enrolled. The incidence of DRI, chest compressions, and administration of epinephrine was 55.9% (532/952), 12.5% (119/952), and 7.0% (67/952), respectively. Multivariate analysis revealed that the risk factors for DRI were GA <28 weeks (odds ratio [OR], 3.147; 95% confidence interval [CI], 2.082–4.755), BW <1000 g (OR, 2.240; 95% CI, 1.606–3.125), and antepartum infection (OR, 1.429; 95% CI, 1.044–1.956). The survival rate was 65.9% (627/952) and was dependent on GA. The rate of BPD was 29.3% (181/627). Multivariate analysis showed that the risk factors for BPD were male (OR, 1.603; 95% CI, 1.061–2.424), DRI (OR, 2.094; 95% CI, 1.328–3.303), respiratory distress syndrome exposed to ≥2 doses of pulmonary surfactants (PS; OR, 2.700; 95% CI, 1.679–4.343), and mechanical ventilation ≥7 days (OR, 4.358; 95% CI, 2.777–6.837). However, a larger BW (OR, 0.998; 95% CI, 0.996–0.999), antenatal steroid (OR, 0.577; 95% CI, 0.379–0.880), and PS use in the delivery room (OR, 0.273; 95% CI, 0.160–0.467) were preventive factors for BPD (all P < 0.05).Conclusion:Improving delivery room resuscitation and management of respiratory complications are imperative during early management of the health of EPI and ELBWI.

<p>Cancer accounts as one of the leading causes of morbidity and mortality. Recent studies focus on the efficiency of phytochemicals in cancer therapy. Influence of quercetin, a flavonoid on the effect of γδ T cells and Wnt/β-catenin signalling pathway in human colon cancer cells (HT55 and HCT116) was investigated. Quercetin at 15-120 µM was observed to markedly reduce the viability of HT55 and HCT116 cells. Quercetin exposure significantly increased γδ T cell proliferation and also raised the expressions of granzyme B (Gra B), perforin (PFP), and interferon- γ (IFN-γ) in γδ T cells. Reduced β-catenin expression with increased expressions of phosphorylated- β-catenin, axin1 and 2 were observed in HT55 and HCT116 cells on exposure to quercetin. However β-actin expression was found to be not much altered. The results suggest that quercetin was able to efficiently potentiate the effect of γδ T cells and modulate Wnt/β-catenin signalling pathway.</p><p> </p>

<p class="Abstract">The main aim of the present research work was to demonstrate the antitumor and apoptotic activities of oxypeucedanin hydrate monoacetate isolated from <em>Angelica dahurica</em> against Caco-2 colon cancer cells. MTT cell viability assay along with clonogenic assays were used to study the effect of the compound on cell cytotoxicity and the colony forming tendency. Fluorescence microscopy using acridine orange/ethidium bromide was used to study the effects on apoptosis. <em>In vitro</em> wound healing assay and Western blotting were carried out to study effect on cell migration and PI3K-signalling pathway. The results revealed that oxypeucedanin hydrate monoacetate inhibited Caco-2 cell proliferation and decreased the number of cancer colony forming cells. This compound exerted anti-migratory effects in dose- and time-dependent manner. As compared to the untreated control, drug-treated cells showed significant inhibition of cancer cell migration. Oxypeucedanin hydrate monoacetate significantly down-regulated the expression of pAkt and pPI3K in Caco-2 cells.</p><p> </p>

Background Common hub genes (CHGs) in gastrointestinal cancers (GICs) and their prognostic potential and immune infiltration in hepatocellular carcinoma (HCC) are limited. Methods Differential analysis of genes in the six GICs was performed, and CHGs were identified using the survival and protein-protein interaction (PPI) network analyses. A polygenic prognostic model was constructed based on the CHGs using the LASSO-Cox regression algorithm and validated in two cohorts. CHGs-related multivariate Cox regression and immune infiltration analyses were performed. Results Ten CHGs (TOP2A, CCNB1, CDK1, MAD2L1, RRM2, CCNA2, BUB1B, CDC6, AURKA, and BUB1) were obtained. In The Cancer Genome Atlas (TCGA) cohort, a 3-gene (CCNB1, MAD2L1, and BUB1) prognostic model was built. A significant difference was showed in the overall survival of patients between the two groups. Moreover, both type I and II interferon response pathways decreased in high-risk HCC patients. These results were also validated in the ICGC and internal cohorts. Conclusions 10 CHGs in six GICs was revealed. In HCC, a prognostic model based on the 3 CHGs in GICs was constructed. Both type I and II interferon response pathways markedly decreased in the high-risk group of HCC patients. This might provide novel perspectives for the targeting and immunotherapy of GICs.