Background: Hair concentrations of antiretrovirals are an innovative and non-invasive method for measuring cumulative antiretroviral exposure and assessing long-term antiretroviral adherence. This study aimed to examine hair concentrations of antiretrovirals in relation to virologic outcomes among PLHIV in Guangxi, China. Methods: Cross-sectional data of hair concentrations of antiretrovirals and HIV viral load were collected from 215 PLHIV in Guangxi, China. Multivariate logistic regression analyses were used to examine the association of hair concentrations of antiretrovirals with virologic outcomes. Results: Of the 215 participants, 215, 67, and 163 PLHIV are receiving lamivudine, zidovudine, and efavirenz, respectively. Multivariate analysis revealed that hair concentrations of lamivudine [odds ratio = 16.52, 95% CI 2.51-108.60, p = 0.004] and efavirenz [odds ratio = 14.26, 95% CI 1.18-172.01, p = 0.036], but not zidovudine [odds ratio = 1.77, 95% CI 0.06-56.14, p = 0.747], were the strongest independent predictor of virologic suppression when controlling for sociodemographic and other HIV-related characteristics. Conclusion:Hair concentrations of lamivudine and efavirenz were the strongest independent predictor of virologic suppression among Chinese PLHIV. Hair analysis of antiretrovirals may provide a non-invasive, cost-effective tool that predicts virologic suppression among PLHIV in China.

The adherence assessment based on the combination of nevirapine (NVP) and its two metabolites (2-hydroxynevirapine and 3-hydroxynevirapine) would more comprehensively and accurately reflect long-term adherence than that of a single prototype. This study aimed to develop a specific, sensitive and selective method for simultaneous detection of the three compounds in hair and explore whether there was consistency among the three compounds in assessing long-term adherence. Furthermore, 75 HIV-positive patients who were taking the NVP drug were randomly recruited and divided into two groups (high-and low-adherence group). All participants self-reported their days of oral drug administration per month and provided their hair strands closest to the scalp at the region of posterior vertex. The concentrations of three compounds in the hair were determined using a developed LC-MS/MS method in multiple reaction monitoring. This method showed good performances in limit of quantification and accuracy with the recoveries from 85 to 115% and in precision with the intra-day and inter-day coefficients of variation within 15% for the three compounds. The population analysis revealed that patients with high-adherence showed significantly higher concentrations than those with low-adherence for all three compounds. There were significantly moderate correlations of nevirapine with 2-hydroxynevirapine and 3-hydroxynevirapin and high correlation between 2-hydroxynevirapine and 3-hydroxynevirapin. The two NVP’s metabolites showed high consistency with NVP in evaluating long-term adherence.

This article is based on the relocation project of the 330 kV overhead line in Xi’an, China. In this paper, the soil settlement under different jacking depths was calculated by using the modified Peck’s formula. Meanwhile, by modeling in ABAQUS, the jacking process of a single-chamber double-line large diameter pipeline under different soil conditions was simulated, and the ground deformation data under the different simulated working conditions were obtained. The results of the two methods were compared with the construction monitoring results, and it was found that the finite element simulation results were closer to the actual results. The control variable method was used in the analysis of the surface soil deformation law to analyze the effect of different soil parameters and pipe jacking depths on surface soil deformation. Finally, the best soil conditions applicable to single-chamber double-line large diameter pipe jacking construction were obtained through comparative analysis. The results show that (1) when using double-line construction, the maximum surface settlement under different soil conditions is located 11–15 m from the centerline of the soil above the pipeline, the minimum settlement location is inside the isolation pile, and with the increase in jacking distance, the settlement at the same section of the surface will gradually decrease and finally produce a small uplift. (2) In the first jacking, the settlement of powder clay is the largest, and the maximum settlement points in the surface section are more distributed. The maximum settlement value is approximately 11.66 mm. The settlement of powder soil is the smallest but produces a certain uplift deformation, and its maximum settlement is more concentrated in the surface section. After the comparison of deformation and soil parameters, loess-like soil is more suitable for single-compartment double-line large diameter pipe jacking construction. (3) When the top pipe burial depth changes, the greater the burial depth is, the smaller the settlement but the greater the lateral influence range. In the soil parameters, the modulus of elasticity only changes 3 MPa, and the settlement change value is approximately 5 mm. By changing the parameters, it can be obtained that the larger the modulus of elasticity of the soil is, the smaller its deformation. The larger the internal friction angle of the soil is, the smaller its deformation, but the maximum value of settlement change is only 1.7 mm, which means that the change in the internal friction angle has little effect on the soil deformation.

To compare the psychological stress level and hair cortisol level of people living with HIV (PLWH) with those without HIV in China, a total of 220 participants were initially enrolled in the study, including 200 PLWH and 20 people living without HIV. Psychological stress level, including quality of life, anxiety, perceived stress and psychological resilience, was self-reported in both groups with related scales. The cortisol in hair was extracted and assessed by LC-APCI-MS/MS method. Propensity score matching analysis was performed to balance the baseline covariates of the two groups, whereas the difference in psychological stress level and hair cortisol level between the two groups was compared. Furthermore, the associations between psychological stress level and cortisol level were examined. Two comparison groups were matched by 1:3 propensity score matching, which yielding 20 people living without HIV and 60 PLWH. Ultimately, in regarding to the psychological stress, the levels of the anxiety (34 vs. 26, p < 0.001), perceived stress (38.5 vs. 33, p = 0.001) and psychological resilience (31 vs. 26, p = 0.004) were higher among PLWH than those living without HIV, but the people without HIV showed higher quality of life (109 vs.116, p < 0. 001). The hair cortisol level (34.66 vs. 21.61, p = 0.002) in PLWH was higher than those living without HIV. However, there were no significant associations between psychological stress level and cortisol level (p > 0.05). The PLWH showed higher level of psychological stress and cortisol than those without HIV. No relationship was seen between psychological stress level and cortisol level in PLWH.

Background:The determination of antiretroviral drugs in hair is receiving considerable research interest to assess long-term adherence to antiretroviral therapy (ART). Currently in China, lamivudine, zidovudine, nevirapine, efavirenz, ritonavir, and lopinavir are combined as first-line and second-line free therapy regimens and are recommended for people living with HIV (PLWH). Simultaneous determination of the 6 antiretroviral drugs in human hair is important for accurately and widely assessing long-term adherence in Chinese PLWH receiving different ART regimens.Methods: Six drugs were extracted from 10-mg hair samples incubated in methanol for 16 hours at 378C and then analyzed by liquid chromatography with tandem mass spectrometry using a mobile phase of 95% methanol, with an electrospray ionization source in multiple reaction monitoring and positive mode. Results:The LC-ESI + -MS/MS method exhibited a linear range (R 2 .