In the present study we address the issue on gut associated lactic acid bacteria (LAB) isolated from the intestine of estuarine fish Mugil cephalus using de Man Rogossa and Sharpe (MRS) agar. LAB isolates were identified biochemically and screened for their ability to inhibit in vitro growth of various fish, shrimp and human pathogens. Most of the LAB isolates displayed an improved antagonism against fish pathogens compared to shrimp and human pathogens. Selected representative strains displaying high antibacterial activity were identified using 16S rRNA gene sequence analysis. Of the selected strains Lactobacillus brevis was the most predominant. Four other species of Lactobacillus, Enterobacter hormaechei and Enterobacter ludwigii were also identified. It was also observed that even among same species, considerable diversity with respect to substrate utilization persisted. Considering the euryhaline nature of grey mullet (Mugil cephalus), the LAB isolated from the gut possessed good tolerance to varying salt concentrations. This finding merits further investigation to evaluate whether the isolated LAB could be used as probiotics in various fresh and sea water aquaculture.

This paper presents an experimental and computational study to assess the potential of plasma actuators to alter the lift of airfoils in view of controlling the output power of wind turbines under high wind conditions. Plasma actuators are electro-fluidic devices that create a jet adjacent to the surface. The proposed concept involves installing an actuator on the blade suction surface pointing in the upstream direction to induce and control boundary layer separation. Experiments have been carried in a subsonic wind tunnel, at angles of attack (AOA) in the range 0–15°, for velocities of 12.60 and 16.30 m/s, on a 6-inch chord untwisted 2–D blade. The aerodynamic balance force measurements show a reduction of lift coefficient for all the AOA. Flow fields obtained by PIV demonstrate that depending of the AOA, the actuator thickens the boundary layer, induces or amplifies its separation. The CFD simulations reproduce the experimental trends. Thus, plasma actuators are potentially efficient devices for lift control over a wind turbine blade.