Abstract:With the continuous development and change of the Times, education view is gradually shifted from teachers to students, and personalized learning begins to return to people's horizons.In this paper, based on CNKI, with co-word visualization analysis as research methods, the personalized learning research hot topic and development trend in our country are summarized and analyzed.Research shows that: the personalized learning research hot topics includes data mining, MOOC, learning environment, learning analysis, ubiquitous learning, learning style, E-learning and teaching mode and so on several aspects;Personalized learning research trend mainly involves the theoretical framework method of personalized learning, the diversification of personalized learning form, the implementation of personalized learning ways and technology and so on several directions.

Purpose: To investigate the distribution and influencing factors of pre-operative conjunctival flora from patients undergoing penetrating ocular surgeries in northeast of China.Methods: An observational and cross-sectional study design was used. In 305 eyes of 305 patients without infective eye diseases who underwent ocular surgeries in the First Affiliated Hospital of Harbin Medical University between May 2018 and May 2019, conjunctival sac scrapings were collected on the day before surgery.Results: The mean age of participants was 60.73 ± 14.23 years, with the majority being female, married and unemployed with at least primary school education. The positive culture rate was 48.20% (147/305 eyes), and a total of 191 bacterial strains were isolated, 22.45% (33/147) positive samples had two or more strains isolated. The most commonly isolated bacteria was Staphylococcus epidermidis (64.92%), surpassing Staphylococcus aureus (5.76%). The culture positive rate of young (18-40 years) group was different between the females(26.67%) and the males(69.23%)(P = 0.024), but in middle aged group and the elderly group, the rates between the sexes were similar with increased trend. Patients visited in summer or autumn presented a higher positive rate than other seasons. The hypertensive women had a higher rate than hypertensive men(58.14% vs. 40%, X2 =5.8662，P = 0.0154). Conclusions: In northeast of China, coagulase-negative Staphylococcus were the most common pre-operative conjunctival bacteria. The hypertensive female patients, elderly patients, or operated patients in summer and autumn should pay more attention to perioperative treatment.Trial registration number：ChiCTR2100044659