In this paper, the key technologies in the construction process of hot in-place recycling were investigated in order to improve the utilization rate of waste asphalt mixture; traditional lab tests including penetration, softening point and ductility tests, atomic force microscope test of recycled asphalt under different rejuvenator content, and the test of milling on grading at different temperatures were carried out. The influence of RAP content and rejuvenation processes on road performance were studied, and the low-temperature performance of mixture was analyzed by the energy analysis method, and the evaluation index was proposed. Test results indicated that the penetration and ductility increases, the softening point decrease with the rejuvenator content increasing, and the optimum rejuvenator content is 4%. The optimum mixing and compaction temperature will decrease by 2–6 °C on average for every 10% increase of RAP content by analyzing the mixture volume index. The results showed enhance rutting resistance of the mixture but lower moisture resistance and low-temperature crack resistance by adding the RAP content. The strain energy density of 10 KJ/m3 is proposed to evaluate the low-temperature performance of the mixture, and 30% RAP produces optimal mixture. The higher rutting resistance and moisture resistance can be obtained by using the construction process of RAP+ rejuvenator co-heating, and higher low-temperature crack resistance with RAP+ rejuvenator without heating.