No abstract

The global burden of cancer have encouraged oncologists to develop novel strategies for treatment. Present study was proposed to develop Arginyl-glycyl-aspartic acid (RGD)-containing nanostructured lipid carriers (NLC) as a delivery system for improving the anticancer capability of epigallocatechin gallate (EGCG) on breast cancer cell line by attaching to integrin superfamily on cancer cells. For this purpose, RGD-containing EGCG-loaded NLC were prepared by hot homogenization technique and characterized by different techniques. Then, cytotoxic and apoptotic effects of prepared nanoparticles and their uptake into cells was evaluated. As results, the nanoparticles with particle size of 85 nm, zeta potential of -21 mV, encapsulation of 83% were prepared. Cytotoxicity and apoptosis experiments demonstrated that EGCG-loaded NLC-RGD possessed greatest apoptotic activity. Furthermore, it has been shown that, EGCG-loaded NLC-RGD causes cell cycle arresting more effective than EGCG. Therefore, loading EGCG into NLC-RGD make it more effective in both targeting and accumulation into tumour cells, which results from specialized uptake mechanism by adhesion to αvβ3 integrin. The results strengthen our hope that loading EGCG into RGD-containing NLC could possibly overcome the therapeutic limitations of EGCG and make it more effective in cancer therapy.

Background: Traditional herbs are effective in the treatment of many diseases. It is proven that traditional herbs can decrease the risk of cancer and grow the survivals of patients. Objectives: Based on the results of our previous research, ethanol Baneh skin extract has cytotoxic effect on pc3 cells; thus, we decide to determine the effect of Baneh extracts on Bcl2 and Bax expression and amount of concentration of caspase3. Methods: The expressions of apoptotic -related genes were determined by real time PCR and the concentration of caspase3 was determined by ELISA method. Results: Ethanol Baneh skin extract increased the mRNA expression of Bax and decreased the mRNA expression of Bcl2 in pc3 cells and also increased the amount of caspase3. Conclusions: The result of this study indicates the Ethanol Baneh skin extract including photochemical that might be a candidate for the herbal anti -cancer drugs.