ABSTRACTa simple, rapid, and with high specificity method has been proposed for the determination of colchicine from biological samples as well from tablet. The method based on the reaction of the colchicine with quaternary ammonium salt in aqueous solution. The analytical parameters such as type of organic solvent (extraction solvent), volume of aqueous solvent, type of salt (ion-pairing agent), concentration of salt and effect pH have been investigated. The calibration graph was linear in the range of 0.3-6 µg mL -1 with the correlation coefficient of 0.999 under the optimum conditions. Good repeatability (R.S.D, %) 2.45, 1.33 and 2.36 for urine and 2.20, 1.97 and 2.79 for plasma in three spiked level 5, 2 and 0.5 µg mL -1 respectively and 2.26 for tablet was obtained.

In the recent years, pollution of the environment by heavy metals has received considerable attention. These elements accumulate in living organisms and are of high toxic potential 1-3. For determination of the trace amount of silver, a separation and a pre-concentration step are usually required before their analysis. This is due to their frequent low concentrations and the matrix effects in the environmental samples 4. However, liquid-liquid extraction (LLE), as the oldest pre-concentration and separation technique in analytical chemistry, is time-consuming and

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