Obesity is a major health problem. We investigated the effects of forskolin and rolipram in the diet of animals in which obesity had been induced. We used 50 female albino Wistar rats that were assigned randomly into five groups as follows: group 1, control; group 2, high fat diet; group 3, high fat diet + forskolin; group 4, high fat diet + rolipram; and group 5, high fat diet + rolipram + forskolin. The rats were fed for 10 weeks and rolipram and forskolin were administered during last two weeks. The animals were sacrificed and blood samples were obtained. Serum cAMP, cGMP and free fatty acids (FFA) levels were measured using ELISA assays. We also measured weight gain during the 10 week period. cAMP and FFA levels of groups 3, 4 and 5 were significantly higher than those of groups 1 and 2. We found no significant differences in serum cGMP levels among the groups. The weight gain in groups 3, 4 and 5 was significantly less than for group 2. We also found that the weight gain in group 5 was significantly less than in groups 3 and 4. We found that both forskolin and rolipram stimulated lipolysis and inhibited body weight increase by increasing cAMP levels. Also, combination therapy using the two agents may be more effective in preventing diet induced obesity than either agent alone. We found also that these agents did not effect cellular cGMP levels in diet induced obesity.

Forskolin ve rolipramın Wistar sıçanlarda diyetle indüklenen obezitede serum leptin, rezistin ve adiponektin seviyeleri üzerine olan etkileri Abstract: Objective: Forskolin, an activator of adenylate cyclase and rolipram a selective inhibitor of phosphodiesterase 4, stimulate lipolysis and inhibit body weight increase by increasing cyclic adenosine monophosphate (cAMP) levels. This study was aimed to investigate the effects of them on leptin, resistin and adiponectin levels in diet induced obesity in rats.Methods: Totally 50 rats were randomly divided into five groups. The Group I was fed with standard pellet diet and the other groups were fed with high-fat diet for 10 weeks. During the last two weeks of the study, group II continued to fed with high-fat diet whereas group III, group IV and group V were administered forskolin, rolipram and forskolin plus rolipram respectively by orogastric tube in addition to their high-fat diet. Then, rats were sacrificed and serum leptin, resistin and adiponectin levels were measured.Results: Although adiponectin levels of group II (p<0.001 for adiponectin, p<0.01 for leptin), group III (p<0.01), group V (p<0.05) were significantly decreased, leptin levels were significantly increased compared to that of the group I. Also, leptin levels of group IV were significantly reduced compared to those of group II (p<0.05). There were no significant differences between resistin levels of the groups.Conclusion: Our results showed that rolipram prevented any alteration in the levels of leptin and adiponectin in addition to its effect on cAMP levels. However, forskolin and rolipram showed no effect on resistin levels of the groups. The underlying mechanism of these findings is not known and needs to be more investigated.Özet: Amaç: Bir adenilat siklaz aktivatörü olan forskolin ve bir selektif fosfodiesteraz 4 inhibitörü olan rolipram siklik adenozin monofosfat (cAMP) seviyesini artırarak lipolizi stimule ve vücut ağırlık artışını inhibe ederler. Bu çalışmada forskolin ve rolipramın sıçanlarda diyetle indüklenen obezitede leptin, rezistin and adiponektin üzerine olan etkilerinin araştırılması amaçlanmıştır.Metod: 50 sıçan randomize olarak 5 gruba ayrıldı. Grup I standart pellet diyet ile diğer gruplar ise yüksek yağlı diyetle 10 hafta beslendi. Çalışmanın son iki haftasında grup II yüksek yağlı diyet ile beslenmeye devam ederken, grup III, grup IV ve grup V'e yüksek yağlı diyete ilave olarak sırası ile forskolin, rolipram ve forskolin ile rolipram orogastrik tüp ile verildi. Sonra sıçanlar sakrifiye edildi ve serum leptin, rezistin and adiponektin seviyeleri ölçüldü. Bulgular: Grup I ile kıyaslandığında Grup II (adiponektin için p<0.001, leptin için p<0.01), grup III (p<0.01) ve İdris Mehmetoğlu et al.: Forskolin and rolipram 235grup V'in (p<0.05) adiponektin seviyeleri anlamlı olarak azalmış, leptin seviyeleri ise anlamlı olarak artmıştı. Grup IV'ün leptin seviyesi grup II ile kıyaslandığında anlamlı olarak azalmıştı (p<0.05). Grupların rezistin seviyeleri arasında anlamlı bir fark bulunama...