The present in vitro study was to ascertain the phytochemicals and the nematicidal potential of organic extract of Datura stramonium and Gongronema latifolium on egg hatch inhibition of M. incognita for the effective control of root knot nematodes. The tested botanical extract are found to possess alkaloids, flavonoids, saponins, steroids, phenols, terpenoids and glycosides in different composition as shown from the phytochemical analysis of the extracts. The egg hatchability test shows that the extracts were very effective in inhibiting egg hatching in all concentrations as compared to the control and the percentage egg hatch inhibition was related to the level of dilution and time of exposure. Datura stramonium was more effective in inhibiting egg hatching of 98% at 100% concentration compared to 76% inhibitory effect of Gongoronema latifolium which may be due to the presence of terpenoids and higher concentration of alkaloids, flavonoids and phenols in the extracts of D. stramolium. The vast bioactive components present in the tested botanicals make them suitable for bio pesticides and nematicide synthesis for eco-friendly and sustainable agro development.