Microorganism in the air is the element of pollution that is the cause of symptoms of various diseases. Microorganisms can be found in all places and survive in bad environmental conditions. One of the pathogenic bacteria found in the air is Staphylococcus aureus. This study aims to determine the effect of using air fresheners containing lemongrass essential oil on the presence of S. aureus bacteria in the house. The rooms of the house that were taken from the air samples included the kitchen, family room, and living room. The design used in this research is the laboratory experiment by using the air capture method. The independent variables observed included the frequency of spraying air freshener and the results were compared with a disinfectant made of 2% sodium hypochlorite solution. The S. aureus bacteria colonies obtained were in the form of golden yellow bacterial colonies, the surface was convex, purple, and cocci-shaped, which indicated the Gram-positive bacteria group. Based on the results of the study, it could be concluded that the use of air fresheners using the active ingredient of lemongrass oil did not significantly affect the decrease in the number of S. aureus bacteria colonies. However, the use of a disinfectant based on sodium hypochlorite solution as a comparison with the spraying frequency three times had an effect on decreasing the number of S.aureus bacteria colonies with the T-test results of sig value 0.007 (P <0.05).