Metabolite profiles and antioxidant activity of Caulerpa racemosa extract with different handlings were investigated. Three different handlings during transportation were applied, namely samples chilled with ice, stored in liquid nitrogen and soaked in seawater. The different handling significantly affected the yield of ethanolic crude extracts and inorganic fractions but insignificantly to organic fractions. Different handlings resulted in differences of major fractions of C. racemosa extracts. Major fractions of the sample that was handled with chilling temperature contained low polar fractions (K 10 , K 11 , K 12 , and K 13 ), while seawater handling extract contained very polar (K 1 , K 2 and K 3 ), polar (K 6 , K 7 , and K 8 ) and low polar (K 13 ) fractions. The extract of the sample handled in liquid nitrogen contained balanced fractions. Chilling temperature handling produced highest antioxidant activity (IC 50 below 2,000 ppm) in ethanolic extract of C. racemosa.