in Wiley InterScience (www.interscience.wiley.com).The magnitude of excess particulate matter separation from a flocculated suspension at upflow settling conditions was predicted from the concentration distribution of batch settling data. The settling data pairs were fitted to modified population-growth model. Settling surfaces at upflow operation were increased by inlaying pipes into the column and inclining it for 508, 608, 70 and 808 coinciding with 0.5563, 0.7152, 1.0455 and 2.0594 mm/s decreased surface hydraulic loading rates, respectively. The respective removal ratios observed at the applied loadings were 0.878, 0.848, 0.756 and 0.520, while the corresponding predicted efficiencies by mean value method were 0.865, 0.831, 0.773 and 0.512, respectively. It was demonstrated that removal predictions obtained from the batch settling tests were quite compatible with the observed removals of actual upflow operation. The investigation also demonstrated that concentration distribution of batch settling data heads successfully to predict the excess particulate matter removal at upflow settling.