Exercise is known to increase the concentrations of irisin, a remarkable myokine that may play an important protective role against metabolic disorders.Propose: This study investigated the effects of irisin signaling pathway induced by Resistance Training (RT) in ovariectomized (Ovx) rats.Methods: Thirty-two Holztman rats were randomly distributed to four experimental groups: Sham-Sedentary (Sed); Ovx-Sed; Sham-RT and Ovx-RT. The RT protocol demanded from the animals a vertical climb. Each session consisted of 4 to 12 climbs with 2 min. of rest during 12 weeks. To quantify mRNA expression the ∆∆Ct method was applied, protein expression was verified by Western Blotting and the analysis of irisin was determined by ELISA. When group averages were different (p ≤ 0.05), a Tukey post-hoc test was applied. Results:The Ovx-RT group had higher expression of PGC1α FNDC5, irisin levels, and UCP1 compared to Ovx-Sed. Conclusion:RT was led to higher expression of the irisin signaling pathway in the Ovx group showing that the RT seems to be an excellent strategy to counteract the ovariectomy-induced metabolic disorders.