RESUMO -Este estudo teve como objetivo avaliar o efeito do regulador de crescimento ácido indolbutírico (AIB) no enraizamento de miniestacas apicais e intermediárias de clones de Eucalyptus urophylla x E. globulus e Eucalyptus grandis x E. globulus. O enraizamento das miniestacas foi conduzido durante 30 dias em casa de vegetação climatizada e, em seguida, as mudas foram transferidas para casa de sombra, permanecendo por 10 dias. O delineamento experimental utilizado foi em blocos ao acaso, em arranjo fatorial triplo, constituído de dois tipos de miniestacas (apicais e intermediárias), três concentrações de AIB (0, 2000 e 4000 mg L -1 ) e 11 clones, em três repetições, compostas de parcelas com 12 miniestacas por repetição. Não houve efeito do AIB sobre as características avaliadas. A sobrevivência e o enraizamento das miniestacas apicais foram significativamente superiores. Houve diferença significativa entre clones quanto às características avaliadas, exceto a sobrevivência após 30 dias na casa de vegetação.Palavras-chave: Propagação clonal, Miniestaquia e Ácido indolbutírico. ROOTING OF MINI-CUTTINGS OF Eucalyptus globulus HYBRID CLONES ABSTRACT -The objective of this study was to evaluate the effect of the growth regulator indol butiric acid (IBA) on the rooting of apical and intermediate mini-cuttings of Eucalyptus urophylla x E. globulus and
Multiplicação in vitro de clones híbridos de... MULTIPLICAÇÃO IN VITRO DE CLONES HÍBRIDOS DE Eucalyptus globulus 1RESUMO -O presente estudo teve como objetivos avaliar a resposta de clones híbridos de Eucalyptus globulus nos meios de cultura MS e JADS durante a fase de multiplicação in vitro. Os explantes foram provenientes da fase de estabelecimento in vitro de 21 clones de Eucalyptus urophylla x E. globulus, sendo 11 clones com três introduções in vitro, e de seis clones de Eucalyptus grandis x E. globulus, sendo dois clones com três introduções. Os clones foram subcultivados mensalmente nos meios de cultura MS e JADS, com 0,5 mg L -1 de BAP (6-benzilaminopurina) e 0,01 mg L -1 de ANA (ácido naftalenoacético). Concluiu-se que: houve tendência de maiores taxas de multiplicação no meio MS; a maioria dos clones se estabeleceu na fase de multiplicação in vitro, enquanto alguns clones se mostraram recalcitrantes nesta fase da micropropagação; a taxa de multiplicação apresentou tendência de aumento nos primeiros subcultivos e posterior queda para a maioria dos clones. IN VITRO MULTIPLICATION OF HYBRID CLONES OF Eucalyptus globulus ABSTRACT -The present study aimed to evaluate the response of hybrid Eucalyptus globulus clones in MS and JADS culture media during the in vitro multiplication phase. The explants were originated from the in vitro establishment phase of 21 Eucalyptus urophylla x E. globulus clones, being 11 clones with three in vitro introductions, and of six Eucalyptus grandis x E. globulus clones, being two clones with three introductions.The clones were subcultivated monthly in MS and JADS culture media,
RESUMO -Cada uma das fases do processo de miniestaquia é essencial para o sucesso da produção de mudas. O presente estudo teve como objetivo avaliar o tempo para o enraizamento em miniestacas de cinco clones híbridos de Eucalyptus grandis e ajustar modelos que melhor expressem o comportamento dos materiais genéticos durante o processo rizogênico. O processo de enraizamento das miniestacas foi conduzido em casa de vegetação climatizada, e em intervalos de tempo regulares de três dias, foram realizadas avaliações do enraizamento adventício, até o 24º dia. Pelas análises, foi possível constatar peculiaridades em relação ao comportamento de cada clone durante o enraizamento e concluir que o tempo necessário para o processo difere entre os materiais genéticos, permitindo a identificação do ponto ótimo de enraizamento das miniestacas.Palavras-chave: Miniestaquia, Clonagem e Silvicultura clonal. OPTIMIZATION OF THE TIME NEEDED FOR MINICUTTINGS ROOTING OF Eucalyptus grandis HYBRID CLONES ABSTRACT -Each phase in the minicutting process is essential for the success of the seedling production. The objective of this study was to assess the time for rooting in minicuttings of five Eucalyptus grandis hybrid clones and to adjust models that best express the behavior of the genetic materials during the rooting process. The minicutting rooting process was carried out in a acclimatized
RESUMO ABSTRACT The present study aimed to evaluate the behavior of Eucalyptus grandis x Eucalyptus globulus andEucalyptus urophylla x Eucalyptus globulus clones and the effect of different in vitro insertions (at 30, 90 and 150 days after pruning the donor plants) in the in vitro establishment phase. The mini-stump plants, explant providers for the in vitro introductions, were cultivated under an indoor semi-hydroponic clonal mini-hedging. Nodal segment explants of 21 Eucalyptus urophylla x Eucalyptus globulus clones and eight Eucalyptus grandis x Eucalyptus globulus clones were harvested, disinfected and inoculated in an MS culture medium, supplemented with 0.5 mg L -1 of BAP and 0.1 mg L -1 of NAA. After 30 days in culture, evaluations were made and it was concluded that there were variations among the clones, as well as in vitro introductions, for the studied variables, where the in vitro establishment was possible for most clones.
Mining activities cause severe impacts to soil, the restoration of which requires specific management, and proper evaluation and monitoring. In this research, our objectives were to study recovery strategies and integrate indicators for monitoring the reclamation of an agricultural soil after bauxite mining. Distinct fertilizations (nonfertilized control [CT], poultry litter [PL], chemical fertilization [CF], and PL + CF combined) and intercrops (bare soil with no intercrops [NI], grass [G], legume [L], and G + L combined) treatments were used as recovery strategies to restore soil capacity to sustain a coffee plantation. We selected 27 quality indicators to compare the premining condition, postmining (reconfigured topsoil), and 19 months after the application of fertilization/intercrops treatments. We used univariate statistics to select soil quality indicators and multivariate analyses to group the selected indicators into organic, chemical, physical, and microbiological properties. From each group, one representative attribute was selected, and its averaged weight was summarized into a soil quality index (SQI). In postmining, the estimated SQI was approximately 65% lower than in premining. The SQI recalculated 19 months after the application of fertilization (PL or PL + CF) and intercrops (G, L, or G + L) was 23% higher than in postmining and showed strong correlation with coffee bean yield (at 27 months). Coffee bean yield was highest in plots with L or G + L receiving PL or PL + CF. We conclude that organic amendments and intercrops are suitable approaches to recover soil following bauxite mining, and soil indicators can be integrated to properly monitor the land reclamation progress.
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