Anas Gilang Pratama, “Analysis of Total Plate Count, Yeast, and Mould Count, Salmonella spp., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella spp. on Traditional Medicines” under the guidance of Silvester Maximus Tulandi, S.Farm., M.Sc., and Ruth Elenora Kristanty, M.Farm., Apt., 2019. Traditional medicines can be contaminated by bacteria starting from the raw material process, during production, and at the time of storage. The analysis of total plate count, yeast, and mould count on traditional medicines was done at the Microbiology Laboratory of National Agency of Drug and Food Control in Jakarta by using five types of bacteria. This work was based on microbiological test parameters on traditional medicines through several stages: homogenization, enrichment, isolation, and biochemical tests. Based on the results, the samples fulfilled the requirements on Analysis of Total Plate Count, Yeast, and Mould Count, Salmonella sp, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella spp. according to the Regulation of the Head of the National Agency of Drug and Food Control No.12/2014.