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We investigated the effects of smoking and exposure to environmental tobacco smoke (ETS) on oxidative DNA damage by measuring 8-oxo-7,8-dihydro-2V -deoxyguanosine (8-oxodGuo) levels in DNA of leukocytes of healthy donors (30 smokers, 29 nonsmokers, and 28 ETS-exposed subjects). Nonsmokers had lower 8-oxodGuo levels compared with smokers (5.94 F 0.87 Â 10 À6 and 19.85 F 4.75 Â 10 À62-deoxyguanosine, respectively, means F SE, P = 0.00007). Subjects exposed to ETS had higher mean value of 8-oxodGuo compared with nonsmokers (9.18 F 1.53 Â 10 À6 2-deoxyguanosine, mean F SE), nonsignificant by univariate analysis (P = 0.074). Multiregression analysis indicated that the increase of 8-oxodGuo levels induced by ETS was significant (P = 0.045) and that coffee and tea consumption reduced DNA oxidation (P = 0.0053). Oxidative leukocyte DNA damage was positively correlated with plasma cotinine levels in ETS-exposed subjects (r = 0.47, P < 0.01, n = 28) and was increased by age in nonsmokers and ETS-exposed subjects (P = 0.049

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The polyphenolic thiaflavans (I) and (II) display the antioxidant features of catechol-containing flavonoids and/or tocopherols. Furthermore, they behave as protective agents against oxidation damage induced in herring sperm DNA by cumene hydroperoxide or by the glutathione/ferric ion (GSH/Fe 3+ ) system. Some of the compounds reveal a protection behavior which is better than that of catechin and α-tocopherol, explainable by both the scavenging properties and chelation of Fe 2+ ions. -(LODOVICI*, M.; MENICHETTI, S.; VIGLIANISI, C.; CALDINI, S.; GIULIANI, E.; Bioorg.