In Integrated Pest Management (IPM), it is sought that the biocides used are to a greater extent selective with the natural enemies present in the field. Within this framework, Ruta graveolens "rue" (Rutaceae) is used for pest control because of its diverse natural properties. This study aimed to evaluate the sensitivity of two biocontrollers Chrysoperla externa and Chrysoperla carnea (Neuroptera: Chrysopidae) compared to aqueous extract of R. graveolens. They were exposed in tests of static acute toxicity (residual contact), larvae of I instar of both species of -1 lacewings to three doses of aqueous extract (2, 5 and 10g·100mL ) of leaves of rue, in flowering stage of the plant. The results of larval mortality at 24 and 48h of exposure were recorded. The correction of mortality was made by Schneider-Orellis's formula. To calculate the values of NOEC and LOEC were used statistical method ANOVA and Duncan post hoc test (p≤0.05). The results show that both species had similar responses to extract in both periods of exposure, no significant differences between the percentages of mortality in the three concentrations and controls. NOEC values were 10g·100mL and LOEC> 10g·100mL , which could suggest the compatibility of these two biocontrollers with the application of aqueous extracts of R. graveolens, and therefore potential use in IPM.