In this work, a systematic approach based on the geometric observer is proposed to design a model-based soft sensor, which allows the estimation of quality indexes in a bioreactor. The study is focused on the structure design problem where the set of innovated states has to be chosen. On the basis of robust exponential estimability arguments, it is found that it is possible to distinguish all the unmeasured states if temperature and dissolved oxygen concentration measurements are combined with substrate concentrations. The proposed estimator structure is then validated through numerical simulation considering two different measurement processor algorithms: the geometric observer and the extended Kalman filter.

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