At the dose and number of sessions used in this study, ozone therapy by rectal insufflation seems to be beneficial for physical symptoms and depression of fibromyalgia.

Introduction. The ozone immune modulation is based on its effect on the immune system and oxidative metabolism. When whole human blood is exposed to the appropriate ozone doses, we can observe in the cells different biochemical reactions that in turn are able to improve the immunity mechanism. Objectives. The aim of this experimental study was to evaluate the effect of ozone on blood phagocytosis. Methods. Blood samples of 30 mL were taken from five healthy male blood donors in the morning, and were subdivided in 6 glass tubes of 5 mL each (control, oxygen and 4 ozone groups). A volume of 5 mL ozone/oxygen mixture at different ozone concentrations (10, 20, 40 and 80 µg/mL) was collected with glass syringes. There after the gas was immediately bubbled in the blood samples. Syringes were slowly but continuously mixed in shaker with stirring, during 10 min allowing a complete mixing of the liquid–gas phases with minimal foaming. Two of the above blood samples were used as controls: the first sample blood and the second blood treated with oxygen only. Phagocytic cell function was assessed by direct microscopic Baehner method. The results were analyzed by paired T test; significance was defined as a value less than 0.05. Results. After ozonation, phagocytic function increase with significant differences in comparison with controls groups (p=0.04). Respect to the ozone concentrations, the phagocytic function increased at 20 µg/mL and even more at 40 µg/mL, but decrease with 80 µg/mL. However, there were no significant differences in phagocytic function in the blood exposed to 10 µg/mL in comparison with controls. Conclusion. Ozone may stimulate the phagocytic function of the peripheral blood cells in a dose-dependent fashion.

The aim of this work is to study the ozone therapy effect in the selective immunoglobulin A deficiency. Taking into account that ozone is able to stimulate several biological systems, to modulate cytokines levels and to enhance immunoglobulin production, a phase II, controlled and randomized, clinical trial was performed. Forty patients were divided at random into 2 groups of 20 patients each: Ozone – received 42 sessions by rectal insufflations during three months, using scaling doses. Control – received subcutaneous Hebertrans during three months. Patients were evaluated at the beginning and one and six months later. The IgA improved, with significant difference (p=0.04) between both groups. The blood levels of IgG were increased significantly (p=0.03) in the group treated with ozone compared to the control group. Leukocyte activity improved significantly (p=0.04) one month later, achieving 97 % of normal values. The clinical evolution was satisfactory in 87 and 80 % of the children that received ozone and Hebertrans, respectively without side effects reported.

Alveolitis is a reversible infection of alveolar process after tooth extraction. Its etiology is unknown, but there are factors increasing its incidence such as traumatisms, infections, decrease of vascular supply of surrounding bone and general systemic status. Aimed at comparing the efficacy of OLEOZON® (ozonated sunflower oil) with Alvogil, treating alveolitis clinically and microbiologically and determining the degree of patients´ satisfaction and side effects. A controlled, randomized, single.blind, phase III clinical trial was conducted at “Reynold García” Polyclinic, Matanzas municipality (Cuba), between January 2007 and May 2010. The sample included 100 adult patients, aging from 20 to 59 years, with diagnostic criterion of alveolitis; 50 patients in the experimental group, to which were applied OLEOZON® and in the other 50 patients, Alvogil, a conventionally used medication of well-known efficacy. Cures were made every 72 hours as well as many visits as necessary to the dentist’s office. Healing criterion was formation of granulation tissue and pain relief. Patients were recovered with OLEOZON® by 92% and with Alvogil, by 78%, in the third visit, with significant differences between both groups. The majority of patients needed from 2 to 3 visits to the dentist’s office in both groups, though it was observed that there was a greater number of patients recovered in the group treated with OLEOZON® in the second visit, with significant differences regarding the control group. OLEOZON® proved better efficacy than Alvogil. No side effects against the medication under study were observed.

Objective. The aim of this study was to determine the usefulness of ozone therapy in the treatment of Infantile Cerebral Palsy (ICP). Patients and methods. A non-controlled clinical assay was made in the Ozone Research Center (CIO), Havana, Cuba from January 2013 to January 2014. The sample was constituted by patients remitted to pediatrics consultation of CIO, to whom inclusion and exclusion criteria were applied. The study group involved 45 patients, from 1 month of birth to 8 years, with cerebral palsy of hypoxic-ischemic cause. The evaluation criteria were: evolution of the motor disorder according to the Gross Motor Function Classification System (GMFCS) scale, modification of muscle tone (Ashworth modified scale) and response to treatment (O’Brien modified scale). The way of administration was rectal insufflation; concentrations between 15, 20, 25 and 30 mg/L were used, volumes varied according to age, making calculation of the dose of ozone according to kilograms of weight. Cycles of 20 sessions, every 3 months were indicated, until completing 4 in 16 months. Patients were clinically evaluated, according to the scales used, before and after each cycle. Results and Discussion. The best answer to treatment was obtained in the group aged ? 4 years. The variables analyzed showed a significant improvement when the ozone treatment concluded. With respect to the evolution of the motor disorder, in 65 % of cases it improved. In the group of children below 4 years, the response was better in relation to the muscle tone. Response to treatment, according to the relatives’ criteria, was of 70 % of the children with marked improvement in the tone and muscle function. Conclusions. The greatest percentage of patients improved in the evolution of the motor disorder; when the Manual Ability Classification System (MACS) scale was applied, more than half the patients showed an improvement. A high percentage of children get a satisfactory result regarding muscle tone and motor function. No side effects were present in any of the cases during the study.