The current study was conducted at Wasit province, Iraq during the period from 28 October 2020 to 28 December 2020. The study included one hundred stool samples taken from questionable patients of Entamoeba histolytica and Helicobacter pylori. Samples were collected from patient attended to General Hospital of Martyr Fairuz at Hay district , Saeed Health center and Hospital of Al-Kut. All samples have been checked using direct smear lugol's iodine stain for Entamoeba histolytica and dipstick for Helicobacter pylori. The result showed that 40 samples (40%) out of 100 patients were gave positive by rapid test cassette; females 23 (23%) positive samples and males 17(17%). While 14 samples (14%)out of 100 samples were positive for Entamoeba histolytica. The highest infection in females 8(8%) and the lowest in males 6(6%). The result showed 10 samples (10%) out of 100 patients positive for both E. histolytica and H. pylori. The highest infection was recorded in females 6(6%) and the lowest in males 4(4%).The highest infection of H.pylori in age group (39 and more) years ; females recorded 6(6%) and males recorded 6(6%), while the lowest infection in group (>1) years old ; females recorded 1(1%) and males recorded 1(1%). The infection rate of Entamoeba histolytica was highest in age group (1-13) years old ; females was recorded 1(1%) and males was recorded 4(4%) , while the lowest in age group (26-39) years old ; females recorded 1(1%) while males without infection. Infection with both E.histolytica and H.pylori was recorded the highest in group (39 and more) years old ; females was recorded 3(3%) and males was recorded 2(2%) while the group (>1) years old do not recorded infections.