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Supplemental Figure 1. Nutrient gradients and gene expression are reproducible across replicates. The figure shows the profiles of single cell growth rate (left), normalized ptsG expression (middle), and normalized acs expression (right) for wildtype (WT, top) and acs mutant populations (bottom), each line shows average values within a single replicate (six to ten chambers). Note that overall profiles are very similar between replicates, though some are shifted slightly towards higher or lower depth (e.g. dark gray line acs mutant). This is likely due to slight variation in the density of cells, chamber height, or flow pressure that can change how deep glucose can penetrate into the chamber before being depleted.