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Purpose: A sampling scheme may significantly affect the accuracy of a sensor. This study was conducted to investigate the effects of sampling point locations on optical reflectance measurements of Chinese cabbage and kale plant leaves. Methods: Variability and similarity of multiple measurements for different parts of the leaves were compared. Results: The results indicate that the variability between the average and individual reflectance spectra was smaller for the blade part than for the vein part. Furthermore, the reflectance for the blade part over the upper leaf area was greater and more stable than those for the other parts for both the cabbage and kale leaf samples. Conclusions: The results provide guidelines for optical reflectance measurements of Chinese cabbage and kale plants. The effects of the number of sampling points, the number of leaves, and the relationships between optical reflectance and leaf components remain to be investigated in the future.

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