Background and Objective: Alcoholic drink produced traditionally by Maluku-Indonesia is called sopi, if consumed in excessive doses, it causes kidney damage. The ability of pletekan leaf extract (Ruellia tuberosa L.) is used as an alternative pharmacy because it has strong antioxidant activity. This study aimed to determine the expression of TNF-" in the kidney of Wistar rats (Rattus norvegicus L.) exposed to sopi alcoholic beverages after being treated with a pletekan herbal extract (Ruellia tuberosa L.). Materials and Methods: Thirty Wistar rats with an average weight of 200 g, divided by group I control (-) were given demineralized water, group II control (+) only given sopi, group III-V were given sopi 2.5 mL/head/body weight and pletekan leaf extract with a concentration of 5.04 mg/100 mL water, 10.08 mg/100 mL water and 12.12 mg/100 mL water for each group. This treatment is done for 24 days by giving sopi 3 times a week, then taking the kidney organ to make kidney histology. Observation of TNF-" expression by immunohistochemistry methods.Results: Wistar rats (R. norvegicus L.) exposed to sopi had decreased creatinine levels then were treated with ethanol extract of pletekan leaf (R. tuberosa L.) at a dose of 6% (12.12 mg/100 mL water) which was more effective in reducing creatinine levels and decreased the expression of TNF-" kidney Wistar rats. Conclusion: Pletekan leaf (Ruellia tuberosa L.) ethanol extract has the potential to prevent/repair kidney damage which is characterized by a decrease in the expression of TNF-" kidney Wistar rats (Rattus norvegicus L.) in line with an increase in the extract dosage in all treatments of sopi.