Abstract. Despite their partial diagnosis and registration, nosocomial infections

Nowadays, saliva is a subject of growing scientific interest because of its definite advantages as diagnostic medium. The aim of our study was to investigate the diagnostic potential and reliability of messenger RNAs (mRNAs) of selected genes—interleukin-6 (IL-6), matrix metalloproteinase-8 (MMP-8) and glutathione synthetase (GSS)—as salivary markers in children with diagnosed pyelonephritis and to correlate their levels with typical urine para-clinical indicators of the disease. Analysis of the mRNA levels for IL-6, MMP-8 and GSS in 28 children hospitalized with the diagnosis of pyelonephritis was conducted applying the method of quantitative reverse transcription polymerase chain reaction (RT-qPCR). In the study group (n = 28), IL-6 mRNA levels demonstrated 64-fold increase (p < 0.001). MMP-8 and GSS mRNA levels were increased in 12 samples in patients with pyelonephritis 3.27 (p < 0.01) and 1.94 (p < 0.001) times, respectively. We found a strong and significant correlation (p < 0.001) between the investigated mRNA for IL-6 and MMP-8, IL-6 and GSS, MMP-8 and GSS. Moderate degree of correlation was established between IL-6 and the typical para-clinical indicator of leucocytes (0.43, p < 0.05) and between GSS and leucocytes (0.54, p < 0.01). Salivary IL-6, MMP-8 and GSS mRNA levels in combination with urine test analysis could be useful diagnostic tool for the very distributed disorder of pyelonephritis in childhood.

Abstract:Introduction: Several studies point out that dental products, such as fluoride varnishes, are able to promote remineralization and to control the development of the carious process. Purpose: To evaluate the effectiveness of fluoropolymers varnishes used for prophylaxis and non-invasive treatment of initial caries d1, d2 in children 4 to 6 years. Material and Methods: Non-invasive treatment of lesions d1a, d1b and d2. After processing results and highlights of the actual study was conducted by the data processing package for mathematical and statistical analysis SPSS v 20.0. Results: In a 4-year-olds observed at (2.7 times higher), and the average lesion before treatment was 11,35 ± 2,41(t=12,43,p<0.001). At 5 and 6 year olds difference before and after treatment was 2.6 times the average value of the lesions prior to treatment with 5 year is 11,25 ± 2,47, and at 6-year was 11,15 ± 2,23 (respectively t = 12,01, p <0,001 for 5 years and t = 12,93, p <0,001 for 6 years). Conclusion: Clinpro ™ White Varnish with TCP (Tri-Calcium phosphate) (3M) is effective in reducing demineralization in subsurface layer.

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