The research project designed for the first time on comparison of Hybrirock seeds and pactol seeds of Brssica napus L. A plant affiliation of Brassicaceae family. The objective of this study was to phytochemically analyzing of France purchased Hybrirock and local pactol seeds by Gas chromatography mass spectrophotometry and comparison of their essential oil components in relation with previous works. The French purchased hybrirock seeds and local pactol seeds were planted in Gerda rasha field Grdarash field, College of Agriculture, Salahaddin University-Erbil and seeda were stored for later chemical analysis. The GC-MS detections showed that the most abundant phytochemical constituents were E, E, 2, 4-Decadienal (22.3% for Hybrirock seed oils and 15.64% for pactol seed) and Hexanal (18.37 for hybrirock seed and 8.72% for Pactol seed oils), followed by 2-Undecenal (7.66% for hybrirock seed and 14.27% for pactol seed oils). Our study demonstrated that both studied genotypes of same species contained different important chemicals and beneficial polyunsaturated fatty acids however glucosinolates were significantly higher in pactol seeds in compare with hybrirock seeds, making pactol seeds less edible and more suitable as nematicides or as precursor of Bio-fuels and the two tested genotype seeds have shown sufficient hydrocarbon content which can be used as a substitute of petrol hydrocarbon. While hybrirock seeds with higher fatty acid contents and lower glucosinolates possessing more edibility features to be used as food products and medical agents.