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Citation of This ArticleMalik A, Syarifdjaya M, Gunawan A, Erlina S, Jaelani A, Wibowo DB: Effect of fish oil addition to the skim milk-egg yolk extender on the quality of frozen-thawed Bali bull spermatozoa. Kafkas Univ Vet Fak Derg, 23 (4): 651-654, 2017651-654, . DOI: 10.9775/kvfd.2017 AbstractThis study was conducted to investigate the effect of fish oil addition to the semen extender on post-thaw quality of Bali bull spermatozoa. Fish oil was added at the doses of 0 (control), 50, 100, 150 and 200 mg/100 mL to the skim milk-egg yolk extender. Supplementation of fish oil at the dose of 100 mg/mL significantly (P<0.05) increased both live sperm rate and motility, but its dose of 200 mg/mL caused a significant (P<0.05) decrease in the live sperm rate when compared with the control group. A significant (P<0.05) increment was observed in abnormality rate of frozen-thawed spermatozoa in comparison with the control group when all doses of fish oil except 50 mg/100 mL were added. In conclusion, addition of 100 mg fish oil to 100 mL of skim milk-egg yolk extender could be beneficial for the improvement of the quality of Bali bull spermatozoa after freeze-thawing. Keywords: Bull semen, Fish oil, Cryopreservation, Live sperm, Motility Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi ÖzetBu çalışma sperma sulandırıcısına katılan balık yağının Bali boğa spermatozoonlarının çözdürme sonrası kalitesi üzerine etkisini araştırmak amacıyla yapıldı. Süt tozu-yumurta sarısı sulandırıcısına 0 (kontrol), 50, 100, 150 ve 200 mg/100 mL dozunda balık yağı ilave edildi. 100 mg/100 mL balık yağı ilavesi kontrol grubu ile karşılaştırıldığında hem canlı sperm oranını hem de motiliteyi önemli derecede (P<0.05) artırdı, ancak 200 mg/100 mL'lik doz canlı sperm oranında önemli (P<0.05) bir azalmaya neden oldu. 50 mg/100 mL'lik doz hariç balık yağının tüm dozları ilave edildiğinde kontrol grubuna kıyasla dondurulmuş-çözdürülmüş spermatozoonların anormallik oranında önemli (P<0.05) bir artış gözlendi. Sonuç olarak, süt tozu-yumurta sarısı sulandırıcısının 100 mL'sine 100 mg balık yağı ilavesi Bali boğa spermatozoonlarının dondurma-çözdürme sonrası kalitesinin artırılması için faydalı olabilir.

The objective of the study was to determine the effect of Moringa oleifera supplementation via Urea Molasses Multi-nutrient moringa Block (UM3B) on consumption and digestibility of dry matter and organic matter in the Bali cattle. A total of 16 Bali cattle aged 2-4 years, of same sex ratio, body weight around 200-250kg was used in this study. The cows were allotted to four equal groups that were placed in individual cages rendered to the distribution of treatment groups. The feed was given in the form of field grass and concentrate. The treatment in the first group (P0) was given UMMB (Urea Molasses Multi-nutrient Block) without moringa, while the second (P1), third (P2) and fourth (P3) groups were given UM3B with 5, 10 and 15% of moringa respectively. The consumption of dry matter and organic matter in the Bali cattle was not significantly different (P>0.05) between control (P0) and all treatment groups. Whereas, the percentage of digestibility of organic matter, as well as, percentage of digestibility of dry matter were significantly different (P<0.05) between control (P0) and treatments of P2 or P3.It is concluded that the consumption, as well as the digestibility of dry matter and organic matter were improved by supplementation of moringa and the best results were obtained at 15% inclusion of moringa in UM3B.