Objective: This study aimed to identify the distribution of M2 macrophage and Treg in Nasopharyngeal Carcinoma (NPC) tumor tissue samples. The presence of these two groups of cells was further correlated to clinical stage, tumor size, the lymphatic node involvement, and metastasis. Methods: The total of 50 formalin-fixed paraffin-embedded (FFPE) NPC tissue samples was collected retrospectively (27 samples) and prospectively (23 samples). Samples were FFPE tissue slices. Immunohistochemistry was done on the FFPE tissue slides using anti-CD-163 and anti-FoxP-3 antibodies for M2 macrophage and Treg detection, respectively. The M2 macrophage interpretation was performed by eye-balling method and the score was divided into 0 (negative), 1 (scant), 2 (focal), and 3 (abundant). The average number of Treg FOXP3+ cells in 5 high power fields (HPF) was calculated. The relationship of M2 macrophage and Treg was tested with Spearman's correlation. The relationship between M2 macrophage and Treg with clinical stage, tumor size, node involvement and metastasis was tested by chi square, with p<0.1. Results: M2 macrophage and Treg were positive correlated (r=0.469, p<0.001). The presence of M2 macrophage and regulatory T cell (Treg) was significantly correlated to tumor size (p= 0.091 for M2 macrophage and p=0.022 for Treg) and clinical stage (p= 0.030 for M2 macrophage and p= 0.002 for Treg), but did not correlate with lymphatic node involvement and metastasis. Conclusions: In Epstein-Barr virus related NPC tumor microenvironment, the presence of M2 macrophage was correlated with Treg, and both types of the cells were correlated with tumor size and clinical stages.

No abstract

Dyslipidemia leads to atherosclerosis which is a risk factor for cardiovascular diseases. Edamame contains omega-3 fatty acids and phytochemical components such as isoflavones, sterols and saponins, and also high in fiber content. Edamame has been proved to have ability to decrease LDL cholesterol level. This research aims to analyze the comparison of edamame flour based low fat enteral formula with commercial low fat formula. This was a descriptive comparatif research design. Data collection was done by doing calculations on nutrient content and bioavailability protein, also organoleptic quality of low fat enteral formula with edamame flour substitution. The result showed that fat content of modified formula was 26.4% of total energy requirement and the value had met dietary requirement for dyslipidemia diet which is not more than <30% of total energy requirement. Type of fat in this formula largely contans unsaturated fats derived from omega-3 fatty acids of soy edamame and omega-9 fatty acids from olive oil. The value of  protein bioavailability which was shown from Amino Acid Score was 32.18, Theoretical Digestibility was 89.27, Net Protein Utilization was 28.73, Protein Worth of Eggs was 16.77, and Protein Eficiency Ratio was 4.51. The result of hedonic quality test showed that the color of low fat enteral formula with edamame flour substitution was light brown, the taste was sweet, the smell was typical edamame aroma, and had liquid consistency. Based on the comparison of commercial enteral formula with non-commercial enteral formula based on edamame flour, it can be concluded that edamame based flour enteral formula can be recommended as a non-commercial enteral formula for patients with dyslipidemia replacing commercial formula.

Head and neck cancer ranks fourth nationally cancer incidence in Indonesia. Oral SCC isone of Head and neck cancer incidence. Oral SCCrelated to several factors, includingsmoking, alcohol, viral infection human papillomavirus (HPV 16/18) and genetic On theother hand, HPV E6 oncoprotein binds and inactivates TP53, and result in loss of control ofthe cell cycle.This study aimed to detect HPV 16/18 infection in oral SCC. Detection of HPVserotypes 16 and 18 performed on FFPE DNA isolates oral SCC with the method of nestedPolymerase Chain Reaction (PCR). Nested PCR was performed in two stages, namelyamplification with L1 primer, followed by specific PCR E6 HPV-16 and HPV-18. A total of33% (11/33) FFPE samples showed positive for HPV 18 infection (single-sized DNA bands415bp) and not detected the presence of infection with HPV 16. It can be concluded that thetype of FFPE biosampel can be used for studies related to HPV infection. Furthermore, itshould be tested on different types biosampel by a larger amount so as to represent theprevalence of oncogenic HPV infection in Indonesia.

Abstract   Background: Purple sweet potato leaves (Ipomoea batatas L.) which has been known as immunomodulatory activity, which contains chemical compounds, such as vitamins, minerals, flavonoids (anthocyanin), especially iron found in a higher proportion in the leaves. Therefore, it is necessary to study the immunomodulatory activity of the leaf extract of purple sweet potato (Ipomoea batatas L.) on the immune response in Balb/c mice who induced by hepatitis B vaccines. Method: Inducing performed at day 0 and day 15. Mice with weights ± 20 g, which has been divided into normal control group, I, II, III, IV and V (each 5 mice), respectively were given orally by the leaf extract of purple sweet potato (Ipomoea batatas L.) (doses 100 mg / kg, 200 mg / kg, 300 mg / kg, 400 mg / kg and 500 mg / kg), normal controls (CMC Na 0,5%). With the volume of giving 1% of body weight in mice, performed during the study (20th days). Blood sampling is done via the tail vein (laeralis vein), to calculate the number of leukocytes (21st days) and lymph decision to count lymphocytes (21st days). Results: The results that the leaf extract of purple sweet potato (ipomoea batatas L.), especially a dose of 500 mg / kg as immunomodulatory activity can increase the number of lymphocytes proliferation cell. Conclusion: Purple sweet potato leaf extract has activity against the increase in the number of lymphocytes cell especially on dose 500 mg/kg BW who induced by hepatitis B vaccine that can stimulated an immune response.