ABSTRAKPenelitian ini bertujuan menentukan frekuensi pemberian vaksin DNA koi herpesvirus (KHV) yang menghasilkan kelangsungan hidup tinggi pada ikan mas yang diinfeksi KHV. Frekuensi pemberian vaksin yaitu vaksinasi satu kali seminggu (K1), vaksinasi dua kali seminggu (K2), vaksinasi tiga kali seminggu (K3), ikan tidak divaksin dan diuji tantang KHV (K4), dan ikan diinjeksi dengan larutan phosphate buffered saline (K5). Pemberian pakan dilakukan 2 kali sehari secara satiasi.Mortalitas pada K1; K2; K3; dan K4 masing-masing adalah 33,33±5,77; 20,00±10,00; 6,67± 5,77; dan 43,33±5,77%, sedangkan kelangsungan hidup relatif (RPS) K1; K2; dan K3 masing-masing adalah 23,07±13,32; 53,84±23,07; 84,60±13,32%. Disimpulkan bahwa pemberian pakan mengandung vaksin DNA anti-KHV dengan frekuensi tiga kali dalam satu minggu efektif meningkatkan kelangsungan hidup ikan mas yang diinfeksi KHV. ____________________________________________________________________________________________________________________ Kata kunci: ikan mas, koi herpesvirus, pakan, vaksin DNA ABSTRACTThis study aims to examine the frequency of koi herpesvirus (KHV) DNA vaccine administration which obtained high survival rate on koi herpesvirus-infected carp. In this study, vaccination was performed by feeding fish with artificial diet containing anti-KHV DNA vaccine. Frequency of vaccine administration tested were vaccination once a week (K1), vaccination twice a week (K2), vaccination three times a week (K3), positive control: the fish were not vaccinated but challenged with KHV (K4), and negative control: fish were injected with phosphate bufferred saline solution (K5). Feeding was performed twice a day at satiation. The mortalily rate on K1, K2, K3, and K4 were 33.33±5.77, 20.00±10.00, 6.67± 5.77, dan 43.33± 5.77 %, respectively. While the survival rate on K1, K2, and K3 were 23.07±13.32, 53.84±23.07, 84.60±13.32 %, respectively. The results showed that vaccination three times a week offered the highest survival rate (84.6 %) and showed higher phagocytic activity compared to the other treatments. Hence, oral vaccination via the feed can effectively increase the survival rate of fish, and this can be as an alternative method to control KHV infection in carp. ____________________________________________________________________________________________________________________