The aim of this study is to determine the content of secondary metabolites ethanol fraction of fruit peel of Limonia acidissima qualitatively and test the activity of antimicrobial on Escherichia coli, Staphylococcus aureus, and Candida albicans. Phytochemical screening was carried out by running for separating the extract in silica gel by eluent n-hexane: ethyl acetate. The Thin Layer Chromatography (TLC) plate results then sprayed with a stain viewer; Alkaloid stain viewer: Dragendorff reagent; Terpenoids: Anisaldehyde-sulfuric acid reagents. The plate is heated to a temperature of 100°C; Flavonoids: H2 SO4 10% reagents; Polyphenols and tannins: FeCl 3 reagents 10%; Anthraquinone: 10% KOH reagent in methanol. The antimicrobial test was carried out using disk diffusion methods with concentrations of 50 mg/ml, 75 mg/ml and 100 mg/ml with control of nystatin and chloramphenicol. Phytochemical screening results of ethanol fraction Limonia acidissima can be detected in the content of alkaloids, terpenoids, anthraquinones, and saponins. Antimicrobial test results obtained data that the ethanol fraction Limonia acidissima has an increasing inhibitory activity of 50 mg/ml, 75 mg/ml, and 100 mg/ml.

A high number of infections from year to year require infectious diseases to get serious attention. The antimicrobial compound exploration must be continued to anticipate the development of infectious diseases. The purpose of this study was to find out in vitro antimicrobial activity of nhexane, ethyl acetate, and ethanol fractions of Piper betle green leaves against S. aureus, and profiles of secondary metabolite compounds contained in these three extracts. The antimicrobial activity test was carried out by disk diffusion test of the fraction of n-hexane, ethyl acetate, and ethanol at a concentration of 6.25, 12.5 and 18.75 µg/disk. Detection of secondary metabolite content was done by the Thin Layer Chromatography method with stain-view reagents. The highest antimicrobial activity was found in the ethyl acetate fraction and was significantly different from the activity in the ethanol and n-hexane fractions. However, the antibacterial activity of all fractions was lower compared to the positive control of amoxiclav 30µg/disk. The phytochemical screening results of secondary metabolites of each fraction were shown that the n-hexane fraction contained alkaloids, terpenoids, flavonoids, polyphenols, and anthraquinone; the ethyl acetate fraction contained terpenoids, polyphenols, and anthraquinone; while the ethanol fraction contained alkaloids, terpenoids, polyphenols, and anthraquinone. Based on the test results, it is concluded that all fractions of Piper betle leaf extract had high antimicrobial activity; meanwhile, the ethyl acetate fraction had the highest activities among others. Each fraction was proven to have a different composition of secondary metabolites.

Abstrak: Pandemi COVID-19 menyebabkan perubahan perilaku untuk selalu menggunakan masker dan hand sanitizer. Upaya preventif yang dilakukan untuk penanggulangan penyebaran covid-19 adalah dengan memberikan edukasi tentang pemanfaatan tanaman obat keluarga (TOGA) yang dapat meningkatkan imunitas tubuh dan sebagai antiseptic. Tujuan kegiatan pengabdian ini adalah meningkatan pengetahuan dan ketrampilan masyarakat melalui transfer ipteks tentang pemanfataan TOGA sebagai minuman kesehatan dan hand sanitizer. Pelaksanaan pengabdian menggunakan 2 metode yaitu penyuluhan dan pelatihan. Mitra yang dilibatkan pada kegiatan ini adalah kelompok Ibu PKK RT 01 RW 10 Kelurahan Bandungrejosari. Jumlah peserta kegiatan sebanyak 20 orang. Monitoring kegiatan dilakukan melalui teknik wawancara sedangkan evaluasi kegiatan melalui penyebaran kuisioner kepada mitra. Kuisioner ini berisi pertanyaan yang berhubungan dengan tingkat pemahaman mitra untuk memanfaatkan TOGA sebagai minuman kesehatan dan hand sanitizer. Kesimpulan hasil kegiatan pengabdian ini adalah Mitra pengabdian memiliki pengetahuan yang baik tentang pembuatan minuman kesehatan bentuk larutan, serbuk effervescent, dan serbuk instan serta pembuatan hand sanitizer. Mitra pengabdian mampu membuat minuman kesehatan bentuk larutan, serbuk effervescent, dan serbuk instan serta hand sanitizer dari tanaman hasil budidaya TOGA yang telah dimiliki sebelumnya. Saran dari kegiatan ini adalah Mitra pengabdian diharapkan tetap menerapkan 5 M dan mengkonsumsi minuman kesehatan untuk mencegah penyebaran COVID-19.Abstract: The COVID-19 pandemic has caused behavioral changes to always use masks and hand sanitizers. Preventive efforts are being taken to prevent the spread of COVID-19 by providing education about the use of TOGA (”Tanaman Obat Keluarga”, Family Medicinal Plants) which can increase the body's immunity and act as antiseptics. The purpose of this service activity is to increase the knowledge and skills of the community through the transfer of science and technology regarding the use of TOGA as a health drink and hand sanitizer. The implementation of service uses 2 methods, namely counseling and training. The partners involved in this activity were the PKK group of RT 01 RW 10, Bandungrejosari Village. The number of participants in the activity was 20 people. Monitoring of activities is carried out through interview techniques, while evaluation of activities is carried out by distributing questionnaires to partners. This questionnaire contains questions about the level of understanding of partners to utilize TOGA as a health drink and hand sanitizer. This service activity concludes that service partners have good knowledge about making health drinks in the form of solutions, effervescent powders, and instant powders as well as making hand sanitizers. Service partners can make health drinks in the form of solutions, effervescent powders, and instant powders as well as hand sanitizers from plants that are the result of TOGA cultivation which has been owned by previous partners. The suggestion from this activity is that service partners are expected to continue to apply the 5 M and consume health drinks to prevent the spread of COVID-19.

Background: Garcinia mangostana Linn (Guttiferae) or commonly know as mangosteen has been

The microorganisms that cause infections can mutate due to excessive antibiotic exposure. One of the new drug search strategies is through the exploration of active ingredients derived from plants that have been used empirically by the community. Moringa oleifera L leaf is a plant that has been used and has been shown to have antibacterial, antifungal, analgesic, and antihypertensive activities. Moringa oleifera L leaves contain secondary metabolites such as alkaloids, tannins, saponins, flavonoids, and phenols. The purpose of this study was to obtain active ingredients from the leaves of Moringa oleifera L which will be used as a Standardized Herbal Medicine product in the treatment of infectious cases. In order to get the active ingredient as an antibacterial, multilevel extraction is carried out using different polarity solvents, so that a fraction containing nonpolar, semipolar and polar compounds will be obtained. Antimicrobial potential will be tested on each fraction using the disk diffusion method. The results of identification of the compounds in the ethyl acetate fraction show the class of compounds Flavonoids, Terpenoids, Polyphenols and Anthraquinone while in the ethanol fraction Moringa oleifera L. leaves show the compounds of the compounds Alkaloids, Flavonoids, Terpenoids, Polyphenols, and Saponin. Antimicrobial activity is shown in the ethyl acetate and ethanol fraction in both Staphylococcus aureus and Escherichia coli.