Abstract. Different extracts containing bioactive components and etheric oil of the flowers of Linum capitatum Kit. (Linacea) of Serbian origin were tested for an antimicrobial activity against four bacteria (Staphylococcus aureus, Escherichia coli, Bacillus subtilus, Pseudomonas aeruginosa), one mold (Aspergillus niger) and one yeast (Candida albicans). The isolated flavonoids were also tested against Staphylococcus aureus, Escherichia coli, Bacillus anhtracis, Pseudomonas aeruginosa, Aspergillus niger, Candida albicans andHerpes simplex virus type.

A phytochemical investigation of the flowers of Linum capitatimi Kit (Linacea) of Serbian origin yielded five additional known Flavonoids including: kaempferol, kaempferol-3-O-galactoside, rutin (quercetin-3-O-rutinosid), genistin (genistein-7-O-glucoside) and orientin (luteolin-8-C-glucoside). The characterization of these compounds was achieved by microanalysis, as well as various Chromatographic and spectroscopic methods ( UV/VIS and 1HNMR)