The Gene H region on raspberry linkage group 2 is an interesting region associated with a number of diverse traits. Cane pubescence is determined by Gene H and cultivars and selections with fine hairs (pubescent canes) are more resistant to cane botrytis and spur blight than non-hairy ones. Gene H is the most significant marker in a QTL on Linkage Group (LG) 2 that control cane pubescence and disease resistances and has also been demonstrated to delay ripening. Gene H phenotype for disease resistance is now used as a visual marker in resistance breeding. This study set out to explain the role of Gene H by characterising gene content in this region through identifying and sequencing BAC clones spanning the region to explain the phenotypic effects on cane hairiness, disease resistance and timing of ripening. This has allowed us to propose that PDF2/GLABROUS2 is Gene H and has also provided an insight into the effects the region has on ripening and disease resistance.