Immunocompetence is known to be controlled by genetics depending on the B-haplotype Major Histocompatibility complex (MHC), for instance and the selection for production traits. Genetic resistance to disease involves many facets of the body's defense system and interactions, and it is extremely complex. Production traits had negative correlation to disease resistant traits.The aim of the current study was to estimate the immune responses against SRBC in selected lines, estimating the genetic trend and genetic correlation between male and female immune responses against SRBC. Humoral response was estimated in the form of titer against sheep RBC, each birds was received an intravenous injection of 0.1 ml of 0.5% suspension of packed SRBC in normal saline at 6 week of age Five day later the blood was collected from Jugular vein of each birds in the individual test tube and sera were collected. In the present investigation, chicks belonging to the selected lines of native chickens were used for the study of immunological traits viz., response to sheep RBCs, IgG concentrations. Finally, 1% sheep RBC suspension was prepared by mixing 1 ml of packed sheep RBCs and 99 ml of PBS which was then used for injection in the birds. The Average means for HA was observed (7.74±0.109) and IgG (1.94±0.72) in native breed. Sex wise HA results showed that male have higher mean (7.925 ± 0.1463) than females (7.57± 0.161) for HA while the same was also estimated for IgG by having mean (2.1194±8.9) and female (1.77±8.18). Test of Homogeneity of Variances was recorded as (0.39) for HA which was significant and IgG level (0.937).

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Poultry is the fastest growing livestock sector in the developing countries. The global poultry sector is expected to continue to grow, as demand for poultry meat is driven by growing needs, population, rising incomes and urbanization. The present experiment was conducted to investigate the effect of inorganic and organic trace minerals supplementation on expression of ChTLR2b gene in broilers. A total of 216 broilers randomly divided into twelve groups and each group consisting of 18 broilers in 3 replicates. T1 group was kept as control. T2, T3 and T4 group was supplemented with zinc (40 mg/kg of feed) inorganic, organic and 50% organic form respectively. T5, T6 and T7 groups was supplemented with selenium (0.3 mg/kg of feed) from inorganic, organic and 50%organic form respectively. T8, T9 and T10 groups was supplemented with chromium (2 mg/kg of feed) from inorganic, organic form and 50% organic form respectively. T11 and T12 group was supplemented with combination of all 3 minerals from inorganic and organic form respectively. RT-PCR expression analysis of ChTLR2b gene in spleen revealed that maximum up regulation (3.8413 fold) was found in T3 group (supplemented with organic Zn @ 40 mg/Kg of feed), followed by T4 (3.5325 fold) whereas in bursa of fabricius the maximum up regulation (2.8921 fold) was found in T6 group followed by T12 (2.5310 fold ) as compared to control group. Upregulation of gene expression ChTLR2b in bursa of fabricius and spleen indicates beneficial effect of organic trace minerals in potentiation of immune system in broilers.

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