In the present investigation, the effect of drying on phytochemical composition of lemongrass powder was evaluated. The lemongrass powder was prepared by traditional (Sun and shade) drying and cabinet drying (45 o C). The qualitative determination of phytochemicals such as alkaloid, flavonoid, tannin, saponin, phenol, carbohydrate and glycoside in aqueous, ethanol, acetone and chloroform extract were carried out. It was revealed that, next to ethanolic extract, aqueous extract showed the presence of rich variety of secondary metabolites. The total phenolic content of cold and hot extract was 2.6 ± 0.02 and 3.8 ± 0.02 mg of gallic acid equivalent/g dw basis. The total flavonoid content in cold and hot percolation were found to be 7.6 ± 0.03 and 8.1 ± 0.03 µg/g of quercetin equivalent dw basis. The alkaloids, saponins, steroids and tannin contents were 1.64 ± 0.05, 1.11 ± 0.05, 0.45 ± 0.05 and 0.93 ± 0.05 g/100 g. The minimum reduction of total phenol content observed in shade drying treatment in cold (15.38%) and hot (13.15%) aqueous extract, whereas maximum reduction in cold (38.46%) and hot (26.31%) extract by cabinet drying. The minimum reduction in flavonoid contents were found in shade drying in cold (3.94%) and hot (3.70%) aqueous extract, whereas maximum reduction in cold (13.15%) and hot (13.58%) aqueous extracts by cabinet drying. The alkaloids, steroid, saponin and tannin recorded their highest values in cabinet drying, i.e., 2.61, 0.72, 2.21 and 2.03 g/100 g, respectively over sun and shade drying.