This study aims to examine the quality of frozen semen in Tris egg yolk (TEY) extender and skimmed milk extender with or witout omega-3. A total of 18 Simmental bulls belong to Lembang Artificial Insemination Centre were divided into three groups. Each was fed with standard feed (R1), standard feeds supplemented with minimal Se and Zn (R2) and standard feed supplemented with maximal of Se and Zn concentration. Semen were collected using an artificial vagina and were evaluated macro- and microscopically. The semen then were divided into four tubes and each diluted with Skimmed, SkimmedOmega 3, TEY or TEY-O. The semen was then packed into a 0.25 ml straw and equilibrated at 5 oC for 4 hours, then frozen above liquid nitrogen vapor, and stored in liquid nitrogen container (-196 oC). The qualities of frozen semen were evaluated on the motility, individual score, viability and integrity of the plasma membrane of sperms. Sperm motility of bulls fed with standard feed (R1) in TEY extender and R3 in TEY and TEY-Omega-3 extender were higher (p <0.05) compared to the other combinations. No difference was found on the individual score. The viability of sperms in bulls fed with standard feed in SkimmedOmega-3 extender was higher than the other treatments and the highest sperm plasma membrane integrity was demonstrated by sperm in bull feeding with R2 in TEY extender.

The objective of this study was to evaluate the use of intravaginal progesterone sponge followed by pregnant mare serum gonadotropin (PMSG) administration at different time of sponge withdrawal on the estrus rate and litter size of Priangan sheep. A total of 33 non-pregnant Priangan sheep were divided into four groups of treatment. Group 1 (G1) received intravaginal sponge containing 30 mg of medroxyprogesterone acetate (MPA). Group 2 (G2), 3 (G3), and 4 (G4) received intravaginal sponge containing 30 MPA and followed by intra muscularly injection of 300 IU PMSG at 24 hours before sponge withdrawal, at sponge withdrawal, and 24 hours after sponge withdrawal, respectively. The result showed that all treatment groups have 100% of estrus rate. However, the occurrence of estrus time varied among the group after intravaginal progesterone sponge withdrawal. Group 1 (G1) and G4 tend to have estrus occurrence at day three after intravaginal progesterone sponge withdrawal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrus occurrence respectively at day 1 (62.5%) and day 2 (50%) after intravaginal progesterone sponge withdrawal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have an 88.9% and 87.5%, respectively. Litter size was significantly reduced in G4. In conclusion, the use of either progesterone alone or combined with PMSG is effective for estrus synchronisation in Priangan sheep, but the administration PMSG at one day after intravaginal progesterone sponge withdrawal reduced the litter size of Priangan sheep. ____________________________________________________________________________________________________________________ Key words: Aceh intravaginal progesterone sponge, litter size, estrus rate, PMSG, sheep ABSTRAK Tujuan dari penelitian ini adalah untuk mengevaluasi perbedaan waktu pencabutan spon progesterone intravagina yang di ikuti pemberian pregnant mare serum gonadotropin (PMSG) terhadap persentase estrus dan jumlah anak per kelahiran domba Priangan. Tiga puluh tiga ekor domba Priangan betina dikelompokkan kedalam empat kelompok perlakuan. Kelompok 1 (K1) adalah kelompok domba yang mendapat perlakuan spon intravagina yang mengandung 30 mg medroxyprogesterone acetate (MPA). Kelompok 2 (K2), 3 (K3), dan 4 (K4) mendapat perlakuan 30 mg MPA yang diikuti permberian 300 IU PMSG secara intramuscular secara berturut-turut pada 24 jam sebelum pencabutan spon, pada waktu pencabutan, dan 24 jam setelah pencabutan spon. Hasil penelitian menunjukkan bahwa 100 % domba pada setiap kelompok menunjukkan gejala estrus. Namun, munculnya waktu estrus setelah pencabutan spon bervariasi antar kelompok perlakuan. K1 dan K4 cenderung menunjukkan waktu estrus pada hari ketiga setelah pencabutan spon dengan respon estrus berturut-turut 55% dan 75%. Waktu estrus pada K2 dan K3 cenderung pada hari kesatu (62,5%) dan hari kedua (50%) secara berturut-turut setelah pencabutan spon. Persentase kebuntingan 100% terjadi pada K2 dan K4, sedangkan persentase kebuntingan pada K1 dan K3 secara berturut...

ABSTRAK Glutathione merupakan antioksidan yang berfungsi melindungi sel dari kerusakan akibat oksigen reaktif (ROS) sehingga dapat mencegah reaksi peroksidasi lipid. Penggunaan glutathione dalam pengencer harus sesuai supaya tidak menimbulkan efek negatif. Penelitian ini bertujuan untuk mengetahui pengaruh level glutathione terhadap kualitas semen kambing Peranakan Etawah post thawing dan mengetahui level glutathione yang menghasilkan kualitas semen yang terbaik. Metode penelitian yang digunakan adalah percobaan laboratorium. Penelitian ini mengunakan rancangan acak kelompok dengan lima kelompok kambing dan lima level glutathione (0, 4, 6, 8 dan 10 mM), setiap perlakuan diulang dua kali. Semen ditampung dengan vagina buatan dan dievaluasi secara makroskopis dan mikroskopis, selanjutnya dilakukan pengolahan menjadi semen beku Data dianalisis dengan analisis ragam dan perbedaan antar perlakuan diuji dengan uji lanjut Duncan. Parameter yang diamati adalah kualitas semen post-thawing meliputi motilitas, abnormalitas, membran plasma utuh dan tudung akrosom utuh (TAU). Hasil penelitian menunjukkan bahwa perlakuan level glutathione berpengaruh nyata (p<0,05) terhadap kualitas semen post-thawing. Hasil uji Duncan menunjukkan bahwa motilitas, TAU dari perlakuan level glutathione 6 mM dan 8 mM tidak berbeda nyata, namun MPU hasil 8 mM nyata lebih tinggi dari level lainnya. Disimpulkan bahwa level glutathione berpengaruh terhadap kualitas semen post-thawing, dan level glutathione 8 mM menghasilkan kualitas semen yang terbaik.Kata kunci: glutathione, kualitas semen, post-thawing, kambing peranakan etawahABSTRACT Glutathione is an antioxidant that functions to protect cells from damage caused by reactive oxygen (ROS) so that it can prevent lipid peroxidation reactions. The use of glutathione in diluents must be suitable so as not to cause negative effects The aim of the research was to knew the effect of glutathione level on semen quality of Etawah Crossbreed goat and to know the level of glutathione that produce the best post-thawed semen quality. This research used grouped randomized design with five groups of goat and five glutathione levels (0, 4, 6, 8, and 10 mM), every treatment was repeated twice. Semen was collected with an artificial vagina and was evaluated as macroscopic and microscopic, they were being processed as frozen semen. Data were analyzed with analysis of variance and differences between treatments were analyzed using the Duncan test. Parameter was observed is pot-thawed semen quality consist of motility, abnormality, intact plasma membrane (IPM) and intact acrosome cup (IAC). The result showed that treatment of glutathione level significantly (p<0.05) affect on post-thawed semen quality. Duncan test showed that motility and IAC from treatment glutathione of 6 mM and 8mM were not significant, nevertheless IPM from treatment 8 mM glutathione significantly higher than other levels. It is concluded that the glutathione level significantly affect on post-thawed semen quality, and 8 mM glutathione level resulted in the best quality.Keywords:  glutathione, semen quality, post-thawed, etawah crossbreed goat

In general, the freezing process of semen will have a detrimental effect on the structure and function of spermatozoa. The critical point in the semen freezing process is when the temperature drops to freezing, but this can be overcome by applying the right equilibration period. This study aimed to find out the best the equilibration length, the freezing rate,  their interaction in the semen freezing process towards membrane sperm cell integrity of the frozen semen post thawing. This study used Priangan sheep’s semen and was carried out according to a completely randomized design with a 3 x 3 split-plot design arrangement. The main plot was equilibration length, i.e., 0.5,  1.5, and 2.5 hours. The subplot was the freezing rate, i.e., 7.5ºC/mins, 13.5ºC/mins, and 19.5ºC/mins. The result of the study showed that there was a significant interaction (P<0.05) between the equilibration length of 1.5 hours and the freezing rate of 13.5°C/mins on the motility and the membrane sperm cell integrity. In conclusion, the best sperm quality was obtained from the equilibration length of 1.5 hours and the freezing rate of 13.5°C/mins.