Introduction Kurdistan Provence west of Iran is the most important habitat of strawberry Fragaria ananassa Duch. in Iran. Strawberry anthracnose disease, caused by Colletotrichum nymphaeae, is one of the main pre-harvest and post-harvest diseases that affect strawberry production in Iran. Several species of Colletotrichum such as C. acutatum J.H Simmonds, C. fragariae Brooks, C. gloeosporioides Penz , and C. nymphaeae Pass. cause strawberry anthracnose disease 1. C. nymphaeae is one of the important pathogens causing strawberry anthracnose diseases in Kurdistan province, Iran. This widespread, aggressive species 2 is a limiting factor in strawberry production. The symptoms appear on the aerial parts of the strawberry plant i.e., stolon, petiole, flower, fruit, leaf, and crown , especially fruit decay such as sunken lesions and conidial ooze on fruits 1. Resistance cultivar to this fungus has not been found and reported in Iran yet. Application of chemical fungicides on fruit against the disease is limited and