To provide insights into the molecular mechanisms underlying diabetes mellitus, we performed a proteomic study on two diabetic animal models, streptozotocin (STZ)-induced diabetic rats (T1DM) and genetically diabetic (C57BL/6J ob/ob) mice (T2DM). To better understand the recovery process of those diabetic rodents, we examined the effect of hypoglycemic dipeptide Cyclo (His-Pro) (CHP) treatment on the differential expression of pancreatic proteins in both animal models. Oral administration of CHP had an excellent hypoglycemic effect in both animal models, lowering the average plasma glucose level by over 50%. Pancreatic proteins were separated by two-dimensional gel electrophoresis (2-DE) and identified by MALDI-TOF mass spectrometry. This study allowed, for the first time, the identification of 34 proteins that are related to diabetes and potential targets of CHP, a potent anti-diabetic agent for both T1DM and T2DM. The alterations in the expression of these proteins could indicate a tendency for diabetic animals to overcome their diabetic state. These proteins are involved in cellular functions such as metabolism, cellular structure, oxidative stress, as well as signal and energy transduction. Some have already been linked to diabetes, suggesting that the newly identified proteins might also be significant in the etiology of this pathology and should be further investigated. Furthermore, CHP has emerged as a potent tool for both the treatment and study of the molecular mechanisms underlying diabetes. Thus, the findings presented here provide new insights into the study and potential treatment of this pathology.

The proteins in plasma perform many important functions in the body, and the protein profiles of the plasma vary under different physiological and pathological conditions. In an attempt to identify novel marker proteins for diabetes prognosis, we examined the effect of hypoglycemic dipeptide cyclo (His-Pro) (CHP) on the differential regulation of plasma proteins in streptozocin-induced diabetic rats and genetically-diabetic (ob/ob) mice. The orally-administrated CHP produced an excellent hypoglycemic effect in both animal models, lowering the average plasma glucose level by over 50 %. In the 2-DE analysis of the plasma, a total of 23 spots among 500 visualized spots were found to be differentially regulated, and they were identified by MALDI/TOF mass spectrometry. These proteins include the down-regulation of Apo E and the up-regulation of FGA, Apo A-I, Apo A-IV, and A1M in STZ-induced diabetic rats. Moreover, CHP significantly reduced the plasma protein levels of FGB, FGC, F12, C1QTNF5, and SPA3K, as well as increased the abundance of A1M, A2M, Apo E, and TTR in genetically-diabetic mice. In conclusion, alteration in the regulation of these proteins indicates that this treatment may be successful in overcoming the diabetic state. The present proteomic data can serve as the basis for the development of specific evidence-based interventions allowing for the prevention and treatment of diabetes.

No abstract

No abstract

Women in the predominantly male oriented college of engineering which is afflicted by the high dropout rate and the low employment rate of coeds in their majoring fields because of the gender sensitivity differences. In order to resolve the related problems Korea government made a WISET(Women in Science Engineering and Technology) program since 2006. And P University has been actively engaged in a movement to raise the gender sensitivity Engineering and to train women engineers for highly qualified work force. As part of the movement a subject called ｢Women sensitive Engineering｣ or ｢Women Sensitivity Ergonomics in daily life｣ that focuses on the gender sensitivity are effective to reduce the dropout rate and to land a job inside their fields. The title of established subject in P university was ｢Women Sensitivity Ergonomics in our Daily Life｣ as well. This study was conducted a survey of the coeds who have completed the ｢Women sensitive Engineering｣ or ｢Women sensitive Ergonomics｣ course to evaluate the degree of satisfaction ergonomics not taken the course. The results showed statistically significant responses indicating that both groups of coeds aware the gender sensitivity differences to Engineering and learned the optimization skill of gender sensitivity ergonomics by this ｢Women sensitive Engineering｣ subject. Also factor analysis for student’s enrollment this new subject was conducted in order to search the student’s satisfaction. This may lay the foundation to offer other major engineering courses for juniors and seniors that emphasize the gender sensitivity differences and ergonomics in Engineering. And we could be expected the decreasing the women students dropout rate from Engineering related fields by studying this subject we develope