This study showed that nasal allergen challenge increased markers of eosinophilic inflammation in both upper and lower airways of children monosensitized to mites, even before the onset of clinical symptoms.

Farklı birçok hastalıkta giderek artan kurkumin kullanımına dair veriler göz önüne alındığında, bu saha çalışmasının amacı, giyardiyazisli buzağılarda enema (proktolizis) ile kurkuminin terapötik etkinliğinin araştırılmasıdır. Gereç ve Yöntemler: Çalışmaya her iki cinsiyette olan, 27-60 günlük yaştaki Giardia duodenalis ile doğal enfekte buzağılar dâhil edildi. Tanı: a) Hızlı ELISA test kiti, b) Mikroskobik ve öncelikli parazitolojik kontrol, c) Asemblaj tayiniyle birlikte Nested Polimeraz Zincir Reaksiyonu analiziyle yapıldı. Giyardiyazisli ve ishalli buzağılar Grup I (2 g/buzağı/gün kurkumin, rektal yolla, 10 gün süreyle) ya da Grup II (herhangi bir ilaç uygulaması yapılmayana kontrol) olmak üzere rastgele 2 gruba ayrıldı. Enema kurkuminin terapötik etkinliği 0., 3., 7. ve 10. günlerde rektal yoldan toplanan dışkı örneklerinin mikroskobik incelemesiyle belirlendi. Bulgular: Gruplar arası kontrol ve sağaltım karşılaştırması 3, 7 ve 10. günlerdeki %58,17 (p=0,125), %99,68 (p=0,001) ve %100 (p=0,001) azalma şeklinde görüldü. Kurkumin enema hiçbir yan etki olmaksızın güvenle uygulanmıştır. Sonuç: Sonuç olarak buzağılarda giyardiyazis sağaltımında enema kurkuminin kullanımının güvenli, kolay ve ekonomik (makul fiyatlanan ve ucuz) olduğu sonucuna varmak yanlış olmayacaktır. Saha uygulamasındaki bu doz rejimi (bu çalışmada model olarak enema kullanıldı) zorlayıcı olmayıp diğer doz planlaması ilerleyen çalışmamızın amacı olacaktır. Enema modeliyle kurkumin kullanımının giyardiyazisli buzağılardaki antiparazitik etkinliği ile kist saçılımında belirgin azalma sağladığı öne sürülebilinir.Anah tar Ke li me ler: Buzağı; giyardiyazis; kurkumin; zerdeçal ABS TRACT Objective: Given the growing cumulative evidence of curcumin usage for several diseases, the purpose of this field study was to detect the therapeutic efficacy of curcumin enema (proctolysis) in calves with giardiasis. Material and Methods: A total of 20 calves naturally infected with Giardia duodenalis at the age of 27 to 60 days, of both sexes were enrolled into the study. The diagnosis was based on a) Rapid diagnostic ELISA test kits, b) Microscopic and prior parasitological check and c) Nested Polymerase Chain Reaction analysis along with assemblage detection. Calves with diarrhea and giardiasis were randomly assigned into 2 groups (n=10), Group I (2 g/calf/day curcumin via rectal route for 10 days) and Group II (were stable as the control without any drug prescription). The therapeutic efficacy of curcumin enema was determined by microscopical examination of faecal samples collected rectally on days 0, 3, 7 and 10. Results: Intra-group comparison of control and treatment calves revealed 58.17% (p=0.125), 99.68% (p=0.001) and 100% (p=0.001) reduction on days 3, 7, and 10, respectively. Curcumin enema was safely used without any side effects. Conclusion: In conclusion, it should not be unwise to draw conclusion that curcumin enema could be safely, easily and economically (reasonably priced and cheap) used for the treatment of giardiasis among calves. At this dose...

Skin microbiota transplantation (SMT) has aroused our interest for several years, with clinical trials. In the present study the purposes were i) to examine the practicability of SMT by Nivea Refining Clear-Up Strips able to transfer unenriched skin microbiota communities and, ii) to analyze the efficacy of SMT on erythema scores comparatively. Material and Methods: Briefly SMT was repeated on days 5 and 12, and the results were compared to initial values. In a total of 4 different healthy anatomical locations of the same case were selected, the strips were placed on moistened skin and allowed them to dry for 10-12 minutes. Then were carefully peeled off, all 4 strips were transferred to the erythema involved skin tissue were also moistured and allowed to attach for at least 12-15 minutes. The skin erythema severity was estimated with visual analogue scale erythema severity score (VAS-ESS) and Atopic Dermatitis Area and Severity Index (ADASI). Results: The Pearson correlations between the factor scores were all above 0.22 and statistically significant (p<0.001) between day 5 ADASI and VAS-ESS scores (r=0.033) and between day 5 ADASI and day 12 VAS-ESS scores (r=0.048, p<0.001). Conclusion:In conclusion by use of SMT ADASI and VAS-ESS scores were decreased. Transfer of skin microbiota between 2 dissimilar autologous microenvironments for each case (at the same host) enrolled would have helped hastening clinical recovery in dogs with atopic dermatitis.

Giardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. The pathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study was to determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis. The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalis between 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and both sexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthy animals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order to determine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopic examination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay device from the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significant changes were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).

In this study, we aimed to investigate whether chronic shift in light/dark cycle alters brain trace element concentrations. For this purpose, 20 male Wistar albino adult rats were weighed and randomly divided into three groups. The first group (n = 6) was the control and had been subjected to 12/12-h light/dark cycle for 30 days. The second group (n = 7) was subjected to 6/18-h light/dark cycle for 15 days, and the third group (n = 7) was also subjected to 6/18-h light/dark cycle for 15 days and then returned to normal 12/12-h light/dark cycle for 15 days. When light/dark cycle protocols were completed, tissue specimens of the frontal lobe, temporal lobe, and brain stem were collected. Iron (Fe), zinc (Zn), and copper (Cu) concentrations of the frontal lobe, temporal lobe, and brain stem were determined by an atomic absorption spectrophotometer. When compared with controls, Fe levels of the temporal lobe significantly increased in 6/18-h light/dark cycle group (p < 0.05), whereas it was statistically unchanged in rats which were exposed to 6/18-h light/dark cycle then returned to the normal 12/12-h light/dark cycle period. Our results show that chronic shift in light/dark cycle affects trace element concentrations of the brain, especially Fe level in the temporal lobe, and these changes are reversible.