This study analyzed raw (unpasteurized) dairy milk samples from farms, milk vendors, and shops in the Peshawar region of Pakistan for the prevalence of pathogenic multidrug-resistant (MDR) Escherichia coli. During August 2018-September 2019, 100 cow 0 s milk samples from 10 subregions of the Town-3 district were tested for the prevalence of E. coli and of Shiga toxin-producing serotype O157: H7 using various biochemical, morphological, and molecular tests. MDR was tested using the disc diffusion method, while Shiga toxin and antibiotic resistance genes were identified by polymerase chain reaction. Twenty eight samples contained E. coli, of which six