Isradipine (ISRA) is a calcium channel blocking agent and a drug of the dihydropyridine (DHP) class. It is utilized for the treatment of hypertension and congestive cardiac failure (CHF). It selectively binds with the calcium channel receptor with high compatibility and averts calcium alteration into cardiac and arterial smooth muscle cells. ISRA employed to cure deliberate hypertension, used alone or in combination with thiazide diuretics. The present review article represents the compilation and discussion of analytical methods published in the literature for estimation of ISRA in pharmaceutical samples and biological matrices consisting of TLC/ densitometry, HPLC, UV-Visible method, Spectrofluorometry, Capillary Electrophoresis (CE), Polarography, Voltammetry and hyphenated techniques such as LC-MS; LC-MS-MS, GC-MS, etc.The anticipated review details about the comparative utilization of various analytical techniques for estimation of ISRA. The present compilation can be essentially explored to potentiate future analytical investigation needs of ISRA.