The first comprehensive checklist of mayflies (Ephemeroptera) of Georgia is provided based on literature data supplemented by our unpublished data, including extensive samples in 2013-2017, works with the collections and types, faunistic observations, and taxonomical contributions. Records of 75 species are provided, with one species reported as a new finding for the country. Notes about the taxonomic status of several species are given. The present contribution represents the first publication of this kind for mayflies within the whole Caucasus area.