Objective. To investigate oxidative stress (OS) and histological changes that occur in the periodontium of subjects with type 2 diabetes mellitus without signs of periodontal disease and to establish if oxidative stress is a possible link between diabetes mellitus and periodontal changes. Materials and Methods. Tissue samples from ten adult patients with type 2 diabetes mellitus (T2D) and eight healthy adults were harvested. The specimens were examined by microscope using standard hematoxylin-eosin stain, at various magnifications, and investigated for tissue levels of malondialdehyde (MDA) and glutathione (GSH). Results. Our results showed that periodontal tissues in patients with T2D present significant inflammation, affecting both epithelial and connective tissues. Mean MDA tissue levels were 3.578 ± 0.60 SD in diabetics versus 0.406 ± 0.27 SD in controls (P < 0.0001), while mean GSH tissue levels were 2.48 ± 1.02 SD in diabetics versus 9.7875 ± 2.42 SD in controls (P < 0.0001). Conclusion. Diabetic subjects had higher MDA levels in their periodontal tissues, suggesting an increased lipid peroxidation in T2D, and decreased GSH tissue levels, suggesting an alteration of the local antioxidant defense mechanism. These results are in concordance with the histological changes that we found in periodontal tissues of diabetic subjects, confirming the hypothesis of OS implication, as a correlation between periodontal disease incidence and T2D.

BackgroundOur aim was to determine whether low level laser therapy (LLLT) can decrease the time between extraction/socket graft and implant placement, by evaluating histological changes in sockets grafted with a particulate allograft material and treated with LLLT.MethodsThirty patients had a socket grafted with a particulate allograft material (MinerOss) covered with a resorbable collagen wound dressing. The patients were then randomly divided into two equal groups (n = 15): test group receiving postoperative LLLT treatment, and control group without postoperative laser treatment. The assessment of bone formation was carried out in both groups at well-determined time intervals after surgery by histostomorphometric analysis.ResultsThe histological results of the site treated with LLLT for 21 days, harvested at 60 days after grafting showed abundant new bone formation without any sign of inflammation. The same results were obtained in the control group not before 120 days post-surgery.ConclusionsIt can be concluded that LLLT photobiomodulation can reduce the healing time after grafting the extraction socket. Histological evidence suggests that new bone formation in the sockets appeared within 60 days after LLLT treatment compared to a minimum of 120 days in the control group.

Objective. For obtaining the perfect balance of a removable complete denture, we have a few clinical procedures to complete, but only the analysis of the occlusal load distribution at various vertical dimensions of occlusion can give us the precise data. Method. The patient selected for this case report was a 35 years old edentulous female who requested prosthetic treatment with complete removable dentures. We followed the usual clinical steps of obtaining an acrylic denture, as well as the T-Scan III analysis during the jaw registration, in different conditions: with the occlusal wax rims, and with the trial denture in the next step, registering three different occlusal vertical dimension -understated, overstated and ideal height. Using these dimensions, we realized four mock-ups, two at the proper vertical dimensions, with various types of tooth color and shape. We analyzed the mock-ups using the T-scan III system. After the dental prosthesis was manufactured, we made another measurement with the T-scan III system. Results. After analyzing all the data from the T-scan III system, we distinguished that the most suitable solution for the patient was the proper dimension mock-up with D2 shade. Conclusions. Although the registered situations were satisfactory, the aesthetic issues are essential, and the functional aspects are fundamental, the most suitable solution in this case, was the mock-up with proper dimensions.

Mass distribution of different infectious diseases appears even more likely in dentistry than other medical specialities. The purpose of the study is to examine different impressions with alginate materials in order to demonstrate the contamination with different pathogens. Material and method. 63 patients were included in this study. They required specific prosthetic treatment. Preliminary impression were obtained with irreversible hydrocolloid material and microbiologically tested shorthy after removal from the mouth, after washed throughly with cold water and after decontamination with specific desinfectants. Results. Microbiologic records on the alginate impressions in different stages, revealed a reduction in number of bacteria, but not completely absence, regardless of the type of decontamination substance. Conclusions. The presence of different pathogens on the surface of irreversible hydrocolloid impresions is certain. There are residual bacteria which differ from one substance to another, irrespective of cleaning stages and decontamination materials used in daily manipulation of dental impresion. Even if time consuming, decontamination of alginate impression is compulsory in dentistry.

Background: The esthetic proportion gauge developed by Chu is using a 78% recurring esthetic dental (RED) proportion and it is based on evaluating the size and proportion of frontal teeth chairside. The aim of the present study was to evaluate the validity of Chu’s device and to measure the correlation between the width and length of the frontal teeth, attempting to identify the application of the proportion gauge in Mureș county. Materials and methods: From the 142 selected patients, 73 fulfilled the inclusion criteria. The height and width values of the participants’ teeth were measured with a digital caliper and evaluated with Chu’s esthetic proportion gauge. The measurements were then compared statistically. Results: The values obtained from the digital caliper measurements did not correlate with Chu’s proportion scale, and the results showed no significant differences between the two sides (p >0.05). Conclusions: In conclusion, the measured teeth ratios did not show a similarity with the predetermined esthetic proportion scale suggested by Chu.