Coatings can protect metallic substrates (e.g., piping, vessels, tubes, etc.) against corrosion attack. The adhesion strength of a coating to its substrate is an essential factor that defines the overall coating integrity and performance. A pull-off adhesion test is commonly performed to determine the coating adhesion strength. This study evaluated the effect of various vital influencing factors on the pull-off adhesion test, including surface preparation of the loading fixture (dolly) and coating surface, types, curing durations and conditions of glue, and scoring of coating around the dolly. Test results were systematically analysed with non-subjective tools such as Image-J software for failure mode assessment and statistical analysis (t-Test) to compare adhesion values.