Objective: The objective of this study was to determine of volatile compounds of essential oils from Cinnamomum sintoc Bl (Sintok) barks belongs to Lauraceae of two districts of Middle Java.Methods: Analysis of essential oil components from the barks of C. sintoc Bl was performed by confirmation of the linear retention indices, following by the comparison of NIST library peak and mass spectrum peak with literature data. Results:The essential oils from Yogyakarta and Jember of C. sintoc Bl obtained by steam distillation with the percentage of 1.10% and 1.15% (w/w), respectively, with eugenol and methyl eugenol having higher percentage, compare to other components, 35-38% and <10%, respectively. The sintok barks oil of Yogyakarta at 0.1 mL a dose decreased the locomotor activity as much as 11.33%, while at 0.3 and 0.5 mL doses increased the locomotor activity as much as 35.83% and 51.13%. Conclusion:It can be concluded from this study that the inhalation of sintok barks oil gave different influence to locomotor activity depends on the doses given.