Robusta coffee processors in Indonesia apply three methods of processing cherry coffee into green coffee beans, which can affect the quality of the coffee produced. Therefore, this study aims to determine the effect of those various processing methods on the coffee beans by finding out differences in Robusta coffee samples’ biochemical content, both as a pulped and green bean. Red-picked Robusta coffee samples were collected from the Kepahiang Bengkulu, one of Indonesia’s highest Robusta coffee producer areas. The primary biochemical contents of coffee, such as moisture, caffeine, chlorogenic acid, sucrose, and lipids, were tested using standard methods on cherry, pulped, and green bean samples. We observed that both pulped and green bean samples showed significant differences (p<0.05) in their biochemical content. Therefore, selecting the right processing method is necessary to get green beans with biochemical content that will produce coffee with preferable sensory attributes.

<p><em>Families are encouraged by Broken Home circumstances marked by parental divorce, or those who have single parents (Single Parent). At times such as the attention and affection of the parents make the child.Jika already stepped on the teenager can cause a crisis that is usually characterized by the tendency of deviant behavior or mischief. Negative reactions caused by adolescents that can damage the physical and personality. This requires the assistance of teachers Guidance and Counseling to develop Guidance and Counseling services in eradicating students' problems as a result of Broken Home.</em><em></em></p><p><strong> </strong></p>

Background: Women who are in pregnancy experience a lot of changes in themselves, both physically and psychologically. Changes continue to occur during the 9 months of pregnancy. Physical and psychological discomfort can be felt from the beginning during pregnancy. Then added with an overview of the labor process and how the baby will be after birth. Approximately 70% of pregnant women experience low back pain (LBP) that may occur since the early trimester, where the peak incidence of LBP occurs in pregnant women in the second and third trimesters of pregnancy. Anxiety or psychological mothers in facing the childbirth process is one of the discomforts experienced by pregnant women.The Aim: This study aims to determine the physical and psychological discomfort of third trimester pregnant women in the PuskesmasBerbahSleman DIY. Method: This was an analytic survey research with a cross sectional design. The research was conducted in the area of PuskesmasBerbahSleman Yogyakarta. The number of samples is 63 TM III pregnant women, the type of data is primary data, questionnaire instrument. Statistical test analysis using Kendal Tau. Results: shows that the age of the respondents is reproductive age (20-35 years) as much as 84.10%, higher education as much as 46%, working mothers as much as 52.40%, a history of spontaneous labor as much as 46%, physical discomfort in the third trimester of pregnancy is moderate. as much as 77.80%, the psychological discomfort of pregnancy in the third trimester was moderate as much as 46%, the anxiety of the third trimester pregnant women due to the Covid-19 pandemic was 52.40%, there was a relationship between physical and psychological discomfort in the third trimester of pregnant women (p-value = 0.012), there is a relationship between physical discomfort and anxiety due to the Covid-19 pandemic in third trimester pregnant women (p-value = 0.013). Conclusion: there is a relationship between physical discomfort between the psychology of pregnant women in the third trimester, there is a relationship between physical discomfort and anxiety due to the Covid-19 pandemic in third trimester pregnant women in the PuskesmasBerbahSleman DIY.

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Hyperinflammation characterized by elevated proinflammatory cytokines known as 'cytokine storms' is the major cause of high severity and mortality seen in COVID-19 patients. The pathology behind the cytokine storms is currently unknown. Increased HMGB1 levels in serum/plasma of COVID-19 patients were reported by many studies, which positively correlated with the level of proinflammatory cytokines. Dead cells following SARS-CoV-2 infection might release a large amount of HMGB1 and RNA of SARS-CoV-2 into extracellular space.HMGB1 is a well-known inflammatory mediator. Additionally, extracellular HMGB1 might interact with SARS-CoV-2 RNA because of its high capability to bind with a wide variety of molecules including nucleic acids and could trigger massive proinflammatory immune responses. This review aimed to critically explore the many possible pathways by which HMGB1-SARS-CoV-2 RNA complexes mediate proinflammatory responses in COVID-19. The contribution of these pathways to impair host immune responses against SARS-CoV-2 infection leading to a cytokine storm was also evaluated. Moreover, since blocking the HMGB1-SARS-CoV-2 RNA interaction might have therapeutic value, some of the HMGB1 antagonists have been reviewed. The HMGB1-SARS-CoV-2 RNA complexes might trigger endocytosis via RAGE which is linked to lysosomal rupture, PRRs activation, and pyroptotic death. High levels of the proinflammatory cytokines produced might suppress many immune cells leading to uncontrolled viral infection and cell damage with more HMGB1 released. Altogether these mechanisms might initiate a proinflammatory cycle leading to a cytokine storm. HMGB1 antagonists could be considered to give benefit in alleviating cytokine storms and serve as a potential candidate for COVID-19 therapy.