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Different pretreatment techniques help to overcome many barriers in achieving desirable bioproducts at low cost as well as at lower energy requirement. This review predominantly aims to summarize all the pretreatment methods for the production of biofuel from biomass. The massive use of fossil fuel led to several huge problems Predominantly, Bioenergy has attained great importance due to rapid depletion in fossil fuel because biofuels are considered to be one of the promising alternatives of nonrenewable sources. The main objective of pretreatment methods is enhancement of sugar production by hydrolyzing the biomass and help in achieving maximum yield. Pretreatment is a significant tool for the conversion of cellulose as well as it is also very important to change the structure of cellulosic biomass. All the major pretreatment techniques are broadly classified into physical, chemical, physicochemical and biological methodologies which includes techniques like Mechanical Comminution, Acid hydrolysis, steam explosion, microwave pretreatment etc. The motive of these pretreatments is to make cellulose more available to the enzymes that assist in the conversion of carbohydrate polymers into fermentable sugars. Biological pretreatments have been analyzed to have immense advantages over physical and chemical pretreatments. Presently, Biomass almost produce about more than 13% of the world's energy. There are many high‐throughput testing ideas and processing technologies which can add up additional values for a better biomass processing in future. Thus, a systemic and computational approach can combine multistage formulation with multistage problem solving so that novel technology can be encouraged for the biorefinery production. This review has done extensive literature study of the various pretreatment technologies which will assist us to produce bioethanols and other biofuels that can be helpful in reducing the adverse effect on the environment. Practical Applications Predominantly, Bioenergy has attained great importance due to rapid depletion in fossil fuel because biofuels are considered to be one of the promising alternatives of nonrenewable sources. Biofuel Production and Food Processing of various pretreatment technologies produce environmentally friendly methods. All methods of pretreatment help to overcome many barriers and achieving desirable products at low cost as well as lower energy requirement with efficient yield. This article assists us to produce bioethanols and other biofuels that can be helpful in reducing the adverse effect on the environment and on the diminishing amount of fossil fuel.